The randomising influence of Brownian motion dominates life on the micrometre scale and confounds microorganisms’ attempts to navigate. Bacterial strategies for overcoming this phenomenon vary depending on the morphology of cells and the nature of the environment in which they swim. We have developed a high-throughput holographic microscope system for tracking several hundreds of cells simultaneously. We image sample volumes up to a cubic millimetre, at capture rates up to 500 Hz. I will discuss how the system operates and outline key results from tracking experiments and the numerical models they inform, in bulk fluids and complex media such as agar.
Period
17 Sept 2020
Event title
58th Annual Meeting of the Biophysical Society of Japan