Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Imaging modality / instrument: Brightfield + Slimfield

Image format: OME TIFF (16 bit) + MicroManager metadata files

Microscope settings:

488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS

Samples and acquisitions:

Fluorescent fusions in live E.coli cells. MMC = mitomycin C





No. fields of view


MMC-


MMC+ (0.5 ug/ml 3h)




RecA-mGFP


7


15




RecB-sfGFP


17


21




MG1655 control


10


10





Approx. size before/after compression: 21 GB / 7 GB