Research output per year
Research output per year
Description
Series GSE175400
We performed bulk RNA-sequencing on expanded clones, derived from single murine LT-HSCs. For each clone, the stem cell population (EPCR+Lin-Sca1+cKit+ [ELSK]) and all remaining cells (nonELSK) were separated by flow sorting and sequenced. In total, we collected and sequenced 11 ELSK and 12 nonELSK samples. Both ELSK and nonELSK groups are further divided by their repopulation outcome - repopulating ELSK (PosELSK), repopulating nonELSK (PosNonELSK), non-repopulating ELSK (NegELSK) and non-repopulating nonELSK (NegNonELSK). We also performed scRNA-seq on hibernating HSCs (n=36) and fetal liver HSCs (n=54).
External deposit with Gene Expression Omnibus (GEO).
We performed bulk RNA-sequencing on expanded clones, derived from single murine LT-HSCs. For each clone, the stem cell population (EPCR+Lin-Sca1+cKit+ [ELSK]) and all remaining cells (nonELSK) were separated by flow sorting and sequenced. In total, we collected and sequenced 11 ELSK and 12 nonELSK samples. Both ELSK and nonELSK groups are further divided by their repopulation outcome - repopulating ELSK (PosELSK), repopulating nonELSK (PosNonELSK), non-repopulating ELSK (NegELSK) and non-repopulating nonELSK (NegNonELSK). We also performed scRNA-seq on hibernating HSCs (n=36) and fetal liver HSCs (n=54).
External deposit with Gene Expression Omnibus (GEO).
| Date made available | 19 Aug 2022 |
|---|---|
| Publisher | Gene Expression Omnibus (GEO) |
Research output
- 1 Article
-
Identification and characterization of in vitro expanded hematopoietic stem cells
Che, J. L. C., Bode, D., Kucinski, I., Cull, A. H., Bain, F., Becker, H. J., Jassinskaja, M., Barile, M., Boyd, G., Belmonte, M., Zeng, A. G. X., Igarashi, K. J., Rubio-Lara, J., Shepherd, M. S., Clay, A., Dick, J. E., Wilkinson, A. C., Nakauchi, H., Yamazaki, S. & Göttgens, B. & 1 others, , 6 Oct 2022, In: EMBO Reports. 23, 10, p. e55502 19 p., e55502.Research output: Contribution to journal › Article › peer-review
Open AccessFile
Projects
- 1 Finished
-
Human Blood Stem Cell Expansion: Empowering New Technology for Stem Cell Medicine
Kent, D. G. (Principal investigator) & Thomas-Oates, J. E. (Co-investigator)
MEDICAL RESEARCH COUNCIL (MRC)
1/10/20 → 30/09/23
Project: Research project (funded) › Research