Tracking ex vivo hematopoietic stem cell function using Fgd5 and EPCR reveals molecular regulators of expansion.



Series GSE175400

We performed bulk RNA-sequencing on expanded clones, derived from single murine LT-HSCs. For each clone, the stem cell population (EPCR+Lin-Sca1+cKit+ [ELSK]) and all remaining cells (nonELSK) were separated by flow sorting and sequenced. In total, we collected and sequenced 11 ELSK and 12 nonELSK samples. Both ELSK and nonELSK groups are further divided by their repopulation outcome - repopulating ELSK (PosELSK), repopulating nonELSK (PosNonELSK), non-repopulating ELSK (NegELSK) and non-repopulating nonELSK (NegNonELSK). We also performed scRNA-seq on hibernating HSCs (n=36) and fetal liver HSCs (n=54).

External deposit with Gene Expression Omnibus (GEO).
Date made available19 Aug 2022
PublisherGene Expression Omnibus (GEO)

Cite this