Videomicroscopy of normal human urothelial cells established in culture as finite (non-immortalized) cell lines and maintained as detailed in (Southgate et al., 1994). Cultures were seeded in 12 well plates and exposed to 100 µM PPADs (pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid) or 0.1% DMSO (as vehicle control) for 10 min, before addition of 0, 10 or 50 μM ATP in replicates of four. The digital videos are then processed using custom cell tracking software implemented using a range of computer vision techniques.
Southgate, J., K. Hutton, D. Thomas, and L.K. Trejdosiewicz. 1994. Normal human urothelial cells in vitro: proliferation and induction of stratification. Laboratory investigation:583-594.
Funded by Wellcome Trust (090577/Z/09/Z) and York Against Cancer.