Adam Wollman

Search results

• 2022

  Critical roles for EGFR and EGFR-HER2 clusters in EGF binding of SW620 human carcinoma cells

  Wollman, A. J. M., Fournier, C., Llorente-Garcia, I., Harriman, O., Payne-Dwyer, A. L., Shashkova, S., Zhou, P., Liu, T-C., Ouaret, D., Wilding, J., Kusumi, A., Bodmer, W. & Leake, M. C., 25 May 2022, In: Journal of the Royal Society Interface. 19, 190, 11 p., 20220088.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Large scale, single-cell FRET-based glucose uptake measurements within heterogeneous populations

  Wollman, A. J. M., Kioumourtzoglou, D., Ward, R., Gould, G. W. & Bryant, N. J., 15 Apr 2022, In: iScience. 25, 4, p. 104023 14 p., 104023.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
• 2021

  Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness

  Jin, X., Lee, J-E., Schaefer, C., Luo, X., Wollman, A. J. M., Payne-Dwyer, A. L., Tian, T., Zhang, X., Chen, X., Li, Y., McLeish, T. C. B., Leake, M. C. & Bai, F., 22 Oct 2021, In: Science Advances. 7, 43, eabh2929.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
• 2020

  EGF signaling in bowel carcinoma cells utilizes higher order architectures of EGFR and HER2

  Wollman, A., Llorente-Garcia, I., Fournier, C., Harriman, O. L., Hargreaves, A., Shashkova, S., Ouaret, D., Zhou, P., Liu, T-C., Wilding, J., Kusumi, A., Bodmer, W. & Leake, M. C., 12 Jul 2020, bioRxiv, at Cold Spring Harbor Laboratory.

  Research output: Working paper › Preprint

• Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiating Bacillus subtilis

  Wollman, A., Muchováb, K., Chromikováb, Z., Wilkinson, A. J., Barak, I. & Leake, M. C., 1 Jun 2020, (Accepted/In press) In: Computational and Structural Biotechnology Journal.

  Research output: Contribution to journal › Article › peer-review

  File
• 2019

  Single-molecule imaging of DNA gyrase activity in living Escherichia coli

  Stracy, M., Wollman, A., Kaja, E., Gapinski, J., Lee, J-E., Leek, V. A., McKie, S. J., Mitchenall, L. A., Maxwell, A., Sherratt, D. J., Leake, M. C. & Zawadzki, P., 10 Jan 2019, In: Nucleic Acids Research. 47, 1, p. 210-220 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome

  Syeda, A. H., Wollman, A., Hargreaves, A. L., Howard, J. A. L., Bruning, J-G., McGlynn, P. & Leake, M. C., 9 Jul 2019, In: Nucleic Acids Research. 47, 12, p. 6287-6298 12 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment

  Sun, Y., Wollman, A., Huang, F., Leake, M. C. & Liu, L-N., 1 Jul 2019, In: The Plant Cell. 31, 7, p. 1648-1664 17 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Staphylococcus aureus toxin LukSF dissociates from its membrane receptor target to enable renewed ligand sequestration

  Haapasalo, K., Wollman, A., de Haas, C., van Kessel, K., van Strijp, J. & Leake, M. C., 1 Mar 2019, In: The FASEB Journal. 33, 3, p. 3807-3824 18 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells

  Wollman, A., Hedlund, E. G., Shashkova, S. & Leake, M. C., 26 Jun 2019, In: Methods. 8 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
• 2018

  Characterising maturation of GFP and mCherry of genomically integrated fusions in Saccharomyces cerevisiae

  Shashkova, S., Wollman, A., Hohmann, S. & Leake, M. C., 20 Jan 2018, In: Bio-protocol. 8, 2, 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• High-speed single-molecule tracking of CXCL13 in the B-Follicle

  Miller, H. L., Cosgrove, J., Wollman, A., O'Toole, P. J., Coles, M. C., Leake, M. C., Taylor, E. & Zhou, Z., 22 May 2018, In: Frontiers in immunology. 9, p. 1073 15 p., 1073.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Molecular coordination of Staphylococcus aureus cell division

  Foster, S., Lund, V. A., Wacnik, K., Turner, R. D., Cotterell, B. E., Walther, C. G., Fenn, S. J., Grein, F., Wollman, A., Leake, M. C., Olivier, N., Cadby, A., Mesnage, S. & Jones, S., 21 Feb 2018, In: eLife. p. 1-31 31 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Single-molecule techniques in biophysics: a review of the progress in methods and applications

  Miller, H. L., Zhou, Z., Shepherd, J. W., Wollman, A. & Leake, M. C., 1 Feb 2018, In: Reports on progress in physics.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
• 2017

  An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor

  Dunn, K. E., Leake, M. C., Wollman, A., Trefzer, M. A., Johnson, S. D. & Tyrrell, A., 22 Mar 2017, In: Royal Society Open Science. 4, 3, 11 p., 160767.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Frequent exchange of the DNA polymerase during bacterial chromosome replication

  Beattie, T. R., Kapadia, N., Nicolas, E., Uphoff, S., Wollman, A., Leake, M. C. & Reyes-Lamothe, R., 31 Mar 2017, In: eLife. 6, 20 p., e21763.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• The yeast Mig1 transcriptional repressor is dephosphorylated by glucosedependent and independent mechanisms

  Shashkova, S., Wollman, A., Leake, M. C. & Hohmann, S., 1 Aug 2017, In: FEMS microbiology letters. 364, 14

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Transcription factor clusters regulate genes in eukaryotic cells

  Wollman, A., Shashkova, S., Hedlund, E. G., Friemann, R., Hohmann, S. & Leake, M. C., 25 Aug 2017, In: eLife. 6, e27451.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
• 2016

  An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy

  Wollman, A. J. M., Miller, H., Foster, S. & Leake, M. C., 17 Oct 2016, In: Physical Biology. 13, 5, 055002.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Designing a single-molecule biophysics tool for characterizing DNA damage for techniques that kill infectious pathogens through DNA damage effects

  Miller, H. L., Wollman, A. & Leake, M. C., 19 May 2016, In: Advances in experimental medicine and biology. 915, p. 115-127

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells

  Leake, M. C. & Wollman, A., 14 Jun 2016, In: Methods in Molecular Biology.

  Research output: Contribution to journal › Article › peer-review
• 2015

  Developing a new biophysical tool to combine magneto-optical tweezers with super-resolution fluorescence microscopy

  Zhou, Z., Miller, H., Wollman, A. & Leake, M., 26 Jun 2015, In: Photonics. 2, 3, p. 758-772 15 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• From Animaculum to single-molecules: 300 years of the light microscope

  Wollman, A. J. M., Nudd, R., Hedlund, E. G. & Leake, M. C., Apr 2015, In: Open Biology. 5, 4, 150019.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time

  Wollman, A. & Leake, M. C., 6 Jul 2015, In: FARADAY DISCUSSIONS. 184, p. 401-424 24 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

• Observing signal transduction directly at the single-molecule level in live eukaryotic cells

  Wollman, A., 2015, Biophysical Society.

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer

  Wollman, A. J. M., Miller, H., Zhou, Z. & Leake, M. C., 1 Apr 2015, In: Biochemical Society transactions. 43, 2, p. 139-145 7 p.

  Research output: Contribution to journal › Article › peer-review

• Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dyes

  Miller, H., Zhou, Z., Wollman, A. J. M. & Leake, M. C., 15 Oct 2015, In: Methods. 88, p. 81-88 8 p.

  Research output: Contribution to journal › Article › peer-review

  File
• 2014

  Observaton of Snf1/Mig1 mediated signal transduction in S. cerevisiæ with high speed single molecule microscopy

  Wollman, A., 2014.

  Research output: Contribution to conference › Poster

• Single-molecule observaton of bacterial replicaton fork pausing

  Wollman, A., 2014.

  Research output: Contribution to conference › Poster

• 2013

  Transport and self-organization across different length scales powered by motor proteins and programmed by DNA

  Wollman, A. J. M., Sanchez-Cano, C., Carstairs, H. M. J., Cross, R. A. & Turberfield, A. J., 10 Nov 2013, In: Nature Nanotechnology. 9, 1, p. 44-7 4 p.

  Research output: Contribution to journal › Article › peer-review