Andrzej Marek Brzozowski

Search results

• 2023

  Non-glycosylated IGF2 prohormones are more mitogenic than native IGF2

  Potalitsyn, P., Mrázková, L., Selicharová, I., Tencerová, M., Ferenčáková, M., Chrudinová, M., Turnovská, T., Brzozowski, A. M., Marek, A., Kaminský, J., Jiráček, J. & Žáková, L., 19 Aug 2023, In: Communications Biology. 6, 1, 15 p., 863.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Structural conservation of insulin/IGF signalling axis at the insulin receptors level in Drosophila and humans

  Viola, C. M., Frittmann, O., Jenkins, H. T., Shafi, T., De Meyts, P. & Brzozowski, A. M., 7 Oct 2023, In: Nature Communications. 14, 1, 13 p., 6271.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2022

  Characterization of insulin crystalline form in isolated β-cell secretory granules

  Asai, S., Moravcová, J., Žáková, L., Selicharová, I., Hadravová, R., Brzozowski, A. M., Nováček, J. & Jiráček, J., 1 Dec 2022, In: Open Biology. 12, 12, 10 p., 220322.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2020

  The efficiency of insulin production and its content in insulin-expressing model β-cells correlate with their Zn 2+ levels: Zn 2+ levels in β-cells

  Dzianová, P., Asai, S., Chrudinová, M., Kosinová, L., Potalitsyn, P., Šácha, P., Hadravová, R., Selicharová, I., Kříž, J., Turkenburg, J. P., Brzozowski, A. M., Jiráček, J. & Žáková, L., 21 Oct 2020, In: Open Biology. 10, 10, 13 p., 200137.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2019

  Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2

  Pompach, P., Viola, C. M., Radosavljević, J., Lin, J., Jiráček, J., Brzozowski, A. M. & Selicharová, I., 9 Oct 2019, In: Frontiers in Endocrinology. 10, p. 1-10 10 p., 695.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2018

  Can Arginine Inhibit Insulin Aggregation? A Combined Protein Crystallography, Capillary Electrophoresis, and Molecular Simulation Study

  Březina, K., Duboué-Dijon, E., Palivec, V., Jiráček, J., Křížek, T., Viola, C. M., Ganderton, T. R., Brzozowski, A. M. & Jungwirth, P., 8 Nov 2018, In: Journal of Physical Chemistry B. 122, 44, p. 10069-10076 8 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Converting Insulin-like Growth Factors 1 and 2 into High-Affinity Ligands for Insulin Receptor Isoform A by the Introduction of an Evolutionarily Divergent Mutation

  Macháčková, K., Chrudinová, M., Radosavljević, J., Potalitsyn, P., Křížková, K., Fábry, M., Selicharová, I., Collinsová, M., Brzozowski, A. M., Žáková, L. & Jiráček, J., 24 Apr 2018, In: Biochemistry. 57, 16, p. 2373-2382 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Site-selective C-C modification of proteins at neutral pH using organocatalyst-mediated cross aldol ligations

  Spears, R. J., Brabham, R. L., Budhadev, D., Keenan, T., McKenna, S. E., Walton, J., Brannigan, J. A., Brzozowski, A. M., Wilkinson, A. J., Plevin, M. J. & Fascione, M. A., 7 Jul 2018, In: Chemical Science. 9, 25, p. 5585-5593 9 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones

  Brzozowski, A. M., 21 Sept 2018, In: Nature Communications. 9, 12 p., 3860 (2018) .

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• The Chlamydia trachomatis PmpD adhesin forms higher order structures through disulphide-mediated covalent interactions

  Paes, W., Dowle, A., Coldwell, J., Leech, A., Ganderton, T. & Brzozowski, A., 18 Jun 2018, In: PLoS ONE. 13, 6, e0198662.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2017

  Computational and Structural Evidence for Neurotransmitter-mediated Modulation of the Oligomeric States of Human Insulin in Storage Granules

  Palivec, V., Viola, C. M., Kozak, M., Ganderton, T. R., Křížková, K., Turkenburg, J. P., Halušková, P., Žáková, L., Jiráček, J., Jungwirth, P. & Brzozowski, A. M., 28 Mar 2017, (E-pub ahead of print) In: The Journal of biological chemistry. 292, 20, p. 8342-8355

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Insulin-like Growth Factor 1 Analogs Clicked in the C Domain: Chemical Synthesis and Biological Activities

  Macháčková, K., Collinsová, M., Chrudinová, M., Selicharová, I., Pícha, J., Buděšínský, M., Vaněk, V., Žáková, L., Brzozowski, A. M. & Jiráček, J., 28 Dec 2017, In: JOURNAL OF MEDICINAL CHEMISTRY. 60, 24, p. 10105-10117 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2016

  Insulin-Insulin-like Growth Factors Hybrids as Molecular Probes of Hormone: Receptor Binding Specificity

  Křížková, K., Chrudinová, M., Povalová, A., Selicharová, I., Collinsová, M., Vaněk, V., Brzozowski, A. M., Jiráček, J. & Zakova, L., 12 May 2016, In: Biochemistry. 55, 21, p. 2903-2913 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Rational steering of insulin binding specificity by intra-chain chemical crosslinking

  Viková, J., Collinsová, M., Kletvíková, E., Budšínský, M., Záková, L., Veverka, V., Hexnerová, R., Aviñó, R. J. T., Straková, J., Selicharová, I., Vank, V., Wright, D. W., Watson, C. J., Turkenburg, J. P., Brzozowski, A. M. & Jiráek, J., 21 Jan 2016, In: Scientific Reports. 6, 12 p., 19431.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice

  Kaye, P., Lacey, C. J. N., Paes, W. N. W., Brzozowski, A. M., Bland, J. M., Brown, N., Reed, S., Carter, D. & Cole, R., 29 Jul 2016, In: Vaccine. 34, 35, p. 4123-4131 9 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2015

  Intramuscular Immunisation with Chlamydial Proteins Induces Chlamydia trachomatis Specific Ocular Antibodies

  Brzozowski, A. M., 26 Oct 2015, In: PLoS ONE. 10, 10, p. 1-13 13 p., e0141209.

  Research output: Contribution to journal › Article › peer-review

  Open Access

• Structural characterization of human heparanase reveals insights into substrate recognition

  Wu, L., Viola, C. M., Brzozowski, A. M. & Davies, G. J., 16 Nov 2015, In: Nature Structural & Molecular Biology. 22, 12, p. 1016-1022 7 p.

  Research output: Contribution to journal › Article › peer-review
• 2014

  Human insulin analogues modified at the B26 site reveal a hormone conformation that is undetected in the receptor complex

  Žáková, L., Kletvíková, E., Lepšík, M., Collinsová, M., Watson, C. J., Turkenburg, J. P., Jiráček, J. & Brzozowski, A. M., 27 Sept 2014, In: Acta Crystallographica. Section D, Biological Crystallography. 70, 10, p. 2765-2774 10 p.

  Research output: Contribution to journal › Article › peer-review

• Insight into the structural and biological relevance of the T/R transition of the N-terminus of the B-chain in human insulin

  Kosinová, L., Veverka, V., Novotná, P., Collinsová, M., Urbanová, M., Moody, N. R., Turkenburg, J. P., Jiráček, J., Brzozowski, A. M. & Žáková, L., 3 Jun 2014, In: Biochemistry. 53, 21, p. 3392-3402 11 p.

  Research output: Contribution to journal › Article › peer-review

• Structural and functional study of the GlnB22-insulin mutant responsible for maturity-onset diabetes of the young

  Křížková, K., Veverka, V., Maletínská, L., Hexnerová, R., Brzozowski, A. M., Jiráček, J. & Žáková, L., 25 Nov 2014, In: PLoS ONE. 9, 11, e112883.

  Research output: Contribution to journal › Article › peer-review

• Thermodynamic and structural investigation of the specific SDS binding of humicola insolens cutinase

  Kold, D., Dauter, Z., Laustsen, A. K., Brzozowski, A. M., Turkenburg, J. P., Nielsen, A. D., Koldsø, H., Petersen, E., Schiøtt, B., De Maria, L., Wilson, K. S., Svendsen, A. & Wimmer, R., Aug 2014, In: Protein science. 23, 8, p. 1023-1035 13 p.

  Research output: Contribution to journal › Article › peer-review
• 2013

  How insulin engages its primary binding site on the insulin receptor

  Menting, J. G., Whittaker, J., Margetts, M. B., Whittaker, L. J., Kong, G. K. -W., Smith, B. J., Watson, C. J., Zakova, L., Kletvikova, E., Jiracek, J., Chan, S. J., Steiner, D. F., Dodson, G. G., Brzozowski, A. M., Weiss, M. A., Ward, C. W. & Lawrence, M. C., 10 Jan 2013, In: Nature. 493, 7431, p. 241-245 5 p.

  Research output: Contribution to journal › Article › peer-review

• Structural Integrity of the B24 Site in Human Insulin is Important for Hormone Functionality

  Zakova, L., Kletvikova, E., Veverka, V., Lepsik, M., Watson, C. J., Turkenburg, J. P., Jiracek, J. & Brzozowski, A. M., 12 Apr 2013, In: Journal of Biological Chemistry. 288, 15, p. 10230-10240 12 p.

  Research output: Contribution to journal › Article › peer-review
• 2012

  Design and Synthesis of Inhibitors of Plasmodium falciparum N-Myristoyltransferase, A Promising Target for Antimalarial Drug Discovery

  Yu, Z., Brannigan, J. A., Moss, D. K., Brzozowski, A. M., Wilkinson, A. J., Holder, A. A., Tate, E. W. & Leatherbarrow, R. J., 25 Oct 2012, In: JOURNAL OF MEDICINAL CHEMISTRY. 55, 20, p. 8879-8890 12 p.

  Research output: Contribution to journal › Article › peer-review

• Insulin and insulin-like growth factor II differentially regulate endocytic sorting and stability of insulin receptor isoform A.

  Morcavallo, A., Genua, M., Palummo, A., Kletvikova, E., Jiracek, J., Brzozowski, A. M., Iozzo, R., Belfiore, A. & Morrione, A., 30 Mar 2012, In: Journal of Biological Chemistry. 287, 14, p. 11422-11436 15 p.

  Research output: Contribution to journal › Article › peer-review
• 2011

  Non-equivalent role of inter- and intra-molecular hydrogen bonds in the insulin dimer interface.

  Antolikova, E., Zakova, L., Turkenburg, J. P., Watson, C. J., Hanclova, I., Sanda, M., Cooper, A., Krause, T., Brzozowski, M. & Jiracek, J., 21 Oct 2011, In: Journal of Biological Chemistry. 286, 42, p. 36968-36977 12 p.

  Research output: Contribution to journal › Article › peer-review
• 2010

  Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF

  Housden, N. G., Wojdyla, J. A., Korczynska, J., Grishkovskaya, I., Kirkpatrick, N., Brzozowski, A. M. & Kleanthous, C., 14 Dec 2010, In: Proceedings of the National Academy of Sciences of the United States of America. 107, 50, p. 21412-21417 6 p.

  Research output: Contribution to journal › Article › peer-review

• Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues

  Jiracek, J., Zakova, L., Antolikova, E., Watson, C. J., Turkenburg, J. P., Dodson, G. G. & Brzozowski, A. M., 2 Feb 2010, In: Proceedings of the National Academy of Sciences of the United States of America. 107, 5, p. 1966-1970 5 p.

  Research output: Contribution to journal › Article › peer-review

• Implications of replacement of amino acids at positions B24 and B26 of insulin by the non-aromatic residues

  Zakova, L. Z., Antolikova, E. A., Brzozowski, A. M. B. & Jiracek, J. J., Sept 2010, In: JOURNAL OF PEPTIDE SCIENCE. 16, p. 162-162 1 p.

  Research output: Contribution to journal › Article › peer-review

• N-Myristoyltransferase from Leishmania donovani: Structural and Functional Characterisation of a Potential Drug Target for Visceral Leishmaniasis

  Brannigan, J. A., Smith, B. A., Yu, Z., Brzozowski, A. M., Hodgkinson, M. R., Maroof, A., Price, H. P., Meier, F., Leatherbarrow, R. J., Tate, E. W., Smith, D. F. & Wilkinson, A. J., 5 Mar 2010, In: Journal of Molecular Biology. 396, 4, p. 985-999 15 p.

  Research output: Contribution to journal › Article › peer-review
• 2009

  Structure of a pullulanase from Bacillus acidopullulyticus

  Turkenburg, J. P., Brzozowski, A. M., Svendsen, A., Borchert, T. V., Davies, G. J. & Wilson, K. S., 1 Aug 2009, In: Proteins - Structure Function and Genetics. 76, 2, p. 516-519 4 p.

  Research output: Contribution to journal › Article › peer-review
• 2008

  A ternary complex of hydroxycinnamoyl-CoA hydratase-lyase (HCHL) with acetyl-CoA and vanillin gives insights into substrate specificity and mechanism

  Bennett, J. P., Bertin, L., Moulton, B., Fairlamb, I. J. S., Brzozowski, A. M., Walton, N. J. & Grogan, G., 1 Sept 2008, In: Biochemical journal. 414, 2, p. 281-289 9 p.

  Research output: Contribution to journal › Article › peer-review

• Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of monoamine oxidase from Aspergillus niger

  Atkin, K. E., Reiss, R., Turner, N. J., Brzozowski, A. M. & Grogan, G., Mar 2008, In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 64, 3, p. 182-185 4 p.

  Research output: Contribution to journal › Article › peer-review

• High-molecular-weight polymers for protein crystallization: poly-gamma-glutamic acid-based precipitants

  Hu, T-C., Korczynska, J., Smith, D. K. & Brzozowski, A. M., 13 Sept 2008, In: Acta Crystallographica. Section D, Biological Crystallography. 64, 9, p. 957-963 7 p.

  Research output: Contribution to journal › Article › peer-review

• The Structure of Monoamine Oxidase from Aspergillus niger Provides a Molecular Context for Improvements in Activity Obtained by Directed Evolution

  Atkin, K. E., Reiss, R., Koehler, V., Bailey, K. R., Hart, S., Turkenburg, J. P., Turner, N. J., Brzozowski, A. M. & Grogan, G., 31 Dec 2008, In: Journal of Molecular Biology. 384, 5, p. 1218-1231 14 p.

  Research output: Contribution to journal › Article › peer-review
• 2007

  Microscale vapour diffusion for protein crystallization

  Korczynska, J., Hu, T-C., Smith, D. K., Jenkins, J., Lewis, R., Edwards, T. & Brzozowski, A. M., Sept 2007, In: Acta Crystallographica. Section D, Biological Crystallography. 63, p. 1009-1015 7 p.

  Research output: Contribution to journal › Article › peer-review

• Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily

  Bennett, J. P., Whittingham, J. L., Brzozowski, A. M., Leonard, P. M. & Grogan, G., 9 Jan 2007, In: Biochemistry. 46, 1, p. 137-144 8 p.

  Research output: Contribution to journal › Article › peer-review

• Structure of the ThDP-dependent enzyme benzaldehyde lyase refined to 1.65 angstrom resolution

  Maraite, A., Schmidt, T., Ansoerge-Schumacher, M. B., Brzozowski, A. M. & Grogan, G., Jul 2007, In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 63, p. 546-548 3 p.

  Research output: Contribution to journal › Article › peer-review

• The use of Fmoc-Lys(Pac)-OH and penicillin G acylase in the preparation of novel semisynthetic insulin analogs

  Zakova, L., Zyka, D., Jezek, J., Hanclova, I., Sanda, M., Brzozowski, A. M. & Jiracek, J., May 2007, In: JOURNAL OF PEPTIDE SCIENCE. 13, 5, p. 334-341 8 p.

  Research output: Contribution to journal › Article › peer-review
• 2006

  Structural and biochemical characterization of a mitochondrial peroxiredoxin from Plasmodium falciparum

  Boucher, I. W., McMillan, P. J., Gabrielsen, M., Akerman, S. E., Brannigan, J. A., Schnick, C., Brzozowski, A. M., Wilkinson, A. J. & Muller, S., Aug 2006, In: Molecular Microbiology. 61, 4, p. 948-959 12 p.

  Research output: Contribution to journal › Article › peer-review

• Structural basis of the sulphate starvation response in E-coli: Crystal structure and mutational analysis of the cofactor-binding domain of the cbl transcriptional regulator

  Stec, E., Witkowska-Zimny, M., Hryniewicz, M. M., Neumann, P., Wilkinson, A. J., Brzozowski, A. M., Verma, C. S., Zaim, J., Wysocki, S. & Bujacz, G. D., 1 Dec 2006, In: Journal of Molecular Biology. 364, 3, p. 309-322 14 p.

  Research output: Contribution to journal › Article › peer-review

• Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases

  Taylor, E. J., Gloster, T. M., Turkenburg, J. P., Vincent, F., Brzozowski, A. M., Dupont, C., Shareck, F., Centeno, M. S. J., Prates, J. A. M., Puchart, V., Ferreira, L. M. A., Fontes, C. M. G. A., Biely, P. & Davies, G. J., 21 Apr 2006, In: Journal of Biological Chemistry. 281, 16, p. 10968-10975 8 p.

  Research output: Contribution to journal › Article › peer-review

• The 1.8 angstrom resolution structure of hydroxycinnamoyl-coenzyme A hydratase-lyase (HCHL) from Pseudomonas fluorescens, an enzyme that catalyses the transformation of feruloyl-coenzyme A to vanillin

  Leonard, P. M., Brzozowski, A. M., Lebedev, A., Marshall, C. M., Smith, D. J., Verma, C. S., Walton, N. J. & Grogan, G., Dec 2006, In: Acta Crystallographica. Section D, Biological Crystallography. 62, p. 1494-1501 8 p.

  Research output: Contribution to journal › Article › peer-review

• The crystal structure of superoxide dismutase from Plasmodium falciparum

  Boucher, I. W., Brzozowski, A. M., Brannigan, J. A., Schnick, C., Smith, D. J., Kyes, S. A. & Wilkinson, A. J., 4 Oct 2006, In: BMC Structural Biology. 6, 20, p. - 10 p.

  Research output: Contribution to journal › Article › peer-review

  File
• 2005

  Structure of a Bacillus halmapalus family 13 alpha-amylase, BHA, in complex with an acarbose-derived nonasaccharide at 2.1 angstrom resolution

  Davies, G. J., Brzozowski, A. M., Dauter, Z., Rasmussen, M. D., Borchert, T. V. & Wilson, K. S., Feb 2005, In: Acta Crystallographica. Section D, Biological Crystallography. 61, p. 190-193 4 p.

  Research output: Contribution to journal › Article › peer-review

• Structures of Plasmodium falciparum purine nucleoside phosphorylase complexed with sulfate and its natural substrate inosine

  Schnick, C., Robien, M. A., Brzozowski, A. M., Dodson, E. J., Murshudov, G. N., Anderson, L., Luft, J. R., Mehlin, C., Hol, W. G. J., Brannigan, J. A. & Wilkinson, A. J., Sept 2005, In: Acta Crystallographica. Section D, Biological Crystallography. 61, p. 1245-1254 10 p.

  Research output: Contribution to journal › Article › peer-review
• 2004

  Crystallization and preliminary crystallographic studies of the cofactor-binding domain of the LysR-type transcriptional regulator Cbl from Escherichia coli

  Stec, E., Witkowska, M., Hryniewicz, M. M., Brzozowski, A. M., Wilkinson, A. J. & Bujacz, G. D., Sept 2004, In: Acta Crystallographica. Section D, Biological Crystallography. 60, p. 1654-1657 4 p.

  Research output: Contribution to journal › Article › peer-review

• The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53

  Krzywda, S., Brzozowski, A. M., Higashitsuji, H., Fujita, J., Welchman, R., Dawson, S., Mayer, R. J. & Wilkinson, A. J., 9 Jan 2004, In: Journal of Biological Chemistry. 279, 2, p. 1541-1545 5 p.

  Research output: Contribution to journal › Article › peer-review

• The interaction of RNA with TRAP: The role of triplet repeats and separating spacer nucleotides

  Hopcroft, N. H., Manfredo, A., Wendt, A. L., Brzozowski, A. M., Gollnick, P. & Antson, A. A., 16 Apr 2004, In: Journal of Molecular Biology. 338, 1, p. 43-53 11 p.

  Research output: Contribution to journal › Article › peer-review

• Toward the insulin-IGF-I intermediate structures: Functional and structural properties of the [Tyr(B25)NMePhe(B26)] insulin mutant

  Zakova, L., Brynda, J., Au-Alvarez, O., Dodson, E. J., Dodson, G. G., Whittingham, J. L. & Brzozowski, A. M., 28 Dec 2004, In: Biochemistry. 43, 51, p. 16293-16300 8 p.

  Research output: Contribution to journal › Article › peer-review