Colin Kleanthous

Search results

• 2018

  Intermembrane crosstalk drives inner membrane protein organization in Escherichia coli

  Rassam, P., Long, K. R., Kaminska, R., Williams, D. J., Papadakos, G., Baumann, C. G. & Kleanthous, K., 14 Mar 2018, In: Nature Communications. 9, 8 p., 1082.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2015

  Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins

  Kleanthous, C., Rassam, P. & Baumann, C. G., 1 Dec 2015, In: CURRENT OPINION IN STRUCTURAL BIOLOGY. 35, p. 109-115 7 p.

  Research output: Contribution to journal › Article › peer-review

• Supramolecular assemblies underpin turnover of outer membrane proteins in bacteria

  Rassam, P., Copeland, N. A., Birkholz, O., Tóth, C., Chavent, M., Duncan, A. L., Cross, S. J., Housden, N. G., Kaminska, R., Seger, U., Quinn, D. M., Garrod, T. J., Sansom, M. S. P., Piehler, J., Baumann, C. G. & Kleanthous, C., 10 Jun 2015, (E-pub ahead of print) In: Nature. 523, p. 333-336 4 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access
• 2012

  Conformational properties of the unfolded state of Im7 in nondenaturing conditions

  Pashley, C. L., Morgan, G. J., Kalverda, A. P., Thompson, G. S., Kleanthous, C. & Radford, S. E., 17 Feb 2012, In: Journal of Molecular Biology. 416, 2, p. 300-18 19 p.

  Research output: Contribution to journal › Article › peer-review

• Kinetic Basis for the Competitive Recruitment of TolB by the Intrinsically Disordered Translocation Domain of Colicin E9

  Papadakos, G., Housden, N. G., Lilly, K. J., Kaminska, R. & Kleanthous, C., 18 May 2012, In: Journal of Molecular Biology. 418, 5, p. 269-280 12 p.

  Research output: Contribution to journal › Article › peer-review

• Structure of the Ultra-High-Affinity Colicin E2 DNase-Im2 Complex

  Wojdyla, J. A., Fleishman, S. J., Baker, D. & Kleanthous, C., 16 Mar 2012, In: Journal of Molecular Biology. 417, 1-2, p. 79-94 16 p.

  Research output: Contribution to journal › Article › peer-review
• 2010

  Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF

  Housden, N. G., Wojdyla, J. A., Korczynska, J., Grishkovskaya, I., Kirkpatrick, N., Brzozowski, A. M. & Kleanthous, C., 14 Dec 2010, In: Proceedings of the National Academy of Sciences of the United States of America. 107, 50, p. 21412-21417 6 p.

  Research output: Contribution to journal › Article › peer-review

• Self-association of TPR domains: Lessons learned from a designed, consensus-based TPR oligomer

  Krachler, A. M., Sharma, A. & Kleanthous, C., Jul 2010, In: Proteins - Structure Function and Genetics. 78, 9, p. 2131-2143 13 p.

  Research output: Contribution to journal › Article › peer-review

• Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3

  Ng, C. L., Lang, K., Meenan, N. A. G., Sharma, A., Kelley, A. C., Kleanthous, C. & Ramakrishnan, V., Oct 2010, In: Nature structural & molecular biology. 17, 10, p. 1241-6 6 p.

  Research output: Contribution to journal › Article › peer-review

• Swimming against the tide: progress and challenges in our understanding of colicin translocation

  Kleanthous, C., Dec 2010, In: Nature reviews. Microbiology. 8, 12, p. 843-848 6 p.

  Research output: Contribution to journal › Article › peer-review

• Thermodynamic dissection of colicin interactions

  Housden, N. G. & Kleanthous, C., 2010, In: Methods in enzymology. 488, C, p. 123-45 23 p.

  Research output: Contribution to journal › Article › peer-review

• The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction

  Meenan, N. A. G., Sharma, A., Fleishman, S. J., MacDonald, C. J., Morel, B., Boetzel, R., Moore, G. R., Baker, D. & Kleanthous, C., 1 Jun 2010, In: Proceedings of the National Academy of Sciences of the United States of America. 107, 22, p. 10080-10085 6 p.

  Research output: Contribution to journal › Article › peer-review

• TolA Modulates the Oligomeric Status of YbgF in the Bacterial Periplasm

  Krachler, A. M., Sharma, A., Cauldwell, A., Papadakos, G. & Kleanthous, C., 22 Oct 2010, In: Journal of Molecular Biology. 403, 2, p. 270-285 16 p.

  Research output: Contribution to journal › Article › peer-review

• Translocator hunt comes full Cir-Col

  Kleanthous, C., Feb 2010, In: Molecular Microbiology. 75, 3, p. 529-33 5 p.

  Research output: Contribution to journal › Article › peer-review
• 2009

  Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins

  Bonsor, D. A., Hecht, O., Vankemmelbeke, M., Sharma, A., Krachler, A. M., Housden, N. G., Lilly, K. J., James, R., Moore, G. R. & Kleanthous, C., 16 Sept 2009, In: The EMBO journal. 28, 18, p. 2846-57 12 p.

  Research output: Contribution to journal › Article › peer-review

• Energy-dependent Immunity Protein Release during tol-dependent Nuclease Colicin Translocation

  Vankemmelbeke, M., Zhang, Y., Moore, G. R., Kleanthous, C., Penfold, C. N. & James, R., 10 Jul 2009, In: Journal of Biological Chemistry. 284, 28, p. 18932-18941 10 p.

  Research output: Contribution to journal › Article › peer-review

• Following evolutionary paths to protein-protein interactions with high affinity and selectivity

  Levin, K. B., Dym, O., Albeck, S., Magdassi, S., Keeble, A. H., Kleanthous, C. & Tawfik, D. S., Oct 2009, In: Nature structural & molecular biology. 16, 10, p. 1049-55 7 p.

  Research output: Contribution to journal › Article › peer-review
• 2008

  A kinetic analysis of regiospecific glucosylation by two glycosyltransferases of Arabidopsis thaliana - Domain swapping to introduce new activities

  Cartwright, A. M., Lim, E.-K., Kleanthous, C. & Bowles, D. J., 6 Jun 2008, In: Journal of Biological Chemistry. 283, 23, p. 15724-15731 8 p.

  Research output: Contribution to journal › Article › peer-review

• Colicin E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on Escherichia coli ribosomes

  Lancaster, L. E., Savelsbergh, A., Kleanthous, C., Wintermeyer, W. & Rodnina, M. V., Jul 2008, In: Molecular Microbiology. 69, 2, p. 390-401 12 p.

  Research output: Contribution to journal › Article › peer-review

• Colicins exploit native disorder to gain cell entry: a hitchhiker's guide to translocation

  Bonsor, D. A., Meenan, N. A. & Kleanthous, C., Dec 2008, In: Biochemical Society transactions. 36, Pt 6, p. 1409-1413 5 p.

  Research output: Contribution to journal › Article › peer-review

• Experimental and computational analyses of the energetic basis for dual recognition of immunity proteins by colicin endonucleases

  Keeble, A. H., Joachimiak, L. A., Mate, M. J., Meenan, N., Kirkpatrick, N., Baker, D. & Kleanthous, C., 13 Jun 2008, In: Journal of Molecular Biology. 379, 4, p. 745-759 15 p.

  Research output: Contribution to journal › Article › peer-review
• 2007

  Analysis of nitroxide spin label motion in a protein-protein complex using multiple frequency EPR spectroscopy

  White, G. F., Ottignon, L., Georgiou, T., Kleanthous, C., Moore, G. R., Thomson, A. J. & Oganesyan, V. S., Apr 2007, In: Journal of Magnetic Resonance. 185, 2, p. 191-203 13 p.

  Research output: Contribution to journal › Article › peer-review

• Biochemical characterization of RAR1 cysteine- and histidine-rich domains (CHORDs): A novel class of zinc-dependent protein-protein interaction modules

  Heise, C. T., Le Duff, C. S., Boter, M., Casais, C., Airey, J. E., Leech, A. P., Amigues, B., Guerois, R., Moore, G. R., Shirasu, K. & Kleanthous, C., 13 Feb 2007, In: Biochemistry. 46, 6, p. 1612-1623 12 p.

  Research output: Contribution to journal › Article › peer-review

• Biophysical characterization of the Self-association of barley sgt1

  Nyarko, A., Mosbahi, K., Shirasu, K. & Kleanthous, C., Jan 2007, In: Biophysical Journal. p. 40A-40A 1 p.

  Research output: Contribution to journal › Article › peer-review

• Colicin biology

  Cascales, E., Buchanan, S. K., Duché, D., Kleanthous, C., Lloubès, R., Postle, K., Riley, M., Slatin, S. & Cavard, D., Mar 2007, In: Microbiology and molecular biology reviews : MMBR. 71, 1, p. 158-229 72 p.

  Research output: Contribution to journal › Article › peer-review

• Molecular mimicry enables competitive recruitment by a natively disordered protein

  Bonsor, D. A., Grishkovskaya, I., Dodson, E. J. & Kleanthous, C., 18 Apr 2007, In: Journal of the American Chemical Society. 129, 15, p. 4800-4807 8 p.

  Research output: Contribution to journal › Article › peer-review

• Structural and functional analysis of SGT1 reveals that its interaction with HSP90 is required for the accumulation of Rx, an R protein involved in plant immunity

  Boter, M., Amigues, B., Peart, J., Breuer, C., Kadota, Y., Casais, C., Moore, G., Kleanthous, C., Ochsenbein, F., Shirasu, K. & Guerois, R., Nov 2007, In: The Plant Cell. 19, 11, p. 3791-3804 14 p.

  Research output: Contribution to journal › Article › peer-review

• The effect of protein complexation on the mechanical stability of Im9

  Hann, E., Kirkpatrick, N., Kleanthous, C., Smith, D. A., Radford, S. E. & Brockwell, D. J., 1 May 2007, In: Biophysical Journal. 92, 9, p. L79-81 3 p.

  Research output: Contribution to journal › Article › peer-review

• The role of electrostatics in colicin nuclease domain translocation into bacterial cells

  Walker, D., Mosbahi, K., Vankemmelbeke, M., James, R. & Kleanthous, C., 26 Oct 2007, In: Journal of Biological Chemistry. 282, 43, p. 31389-31397 9 p.

  Research output: Contribution to journal › Article › peer-review

• TPR-Mediated self-association of plant SGT1

  Nyarko, A., Mosbahi, K., Rowe, A. J., Leech, A., Boter, M., Shirasu, K. & Kleanthous, C., 9 Oct 2007, In: Biochemistry. 46, 40, p. 11331-11341 11 p.

  Research output: Contribution to journal › Article › peer-review
• 2006

  Assignment of the zinc ligands in RsrA, a redox-sensing ZAS protein from Streptomyces coelicolor

  Zdanowski, K., Doughty, P., Jakimowicz, P., O'Hara, L., Buttner, M. J., Paget, M. S. B. & Kleanthous, C., 11 Jul 2006, In: Biochemistry. 45, 27, p. 8294-8300 7 p.

  Research output: Contribution to journal › Article › peer-review

• Calorimetric dissection of colicin DNase-immunity protein complex specificity

  Keeble, A. H., Kirkpatrick, N., Shimizu, S. & Kleanthous, C., 14 Mar 2006, In: Biochemistry. 45, 10, p. 3243-3254 12 p.

  Research output: Contribution to journal › Article › peer-review

• Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9

  Loftus, S. R., Walker, D., Maté, M. J., James, R., Moore, G. R., Kleanthous, C. & Bonsor, D. A., 15 Aug 2006, In: Proceedings of the National Academy of Sciences of the United States of America. 103, 33, p. 12353-12358 6 p.

  Research output: Contribution to journal › Article › peer-review

• Global structural rearrangement of the cell penetrating ribonuclease colicin E3 on interaction with phospholipid membranes

  Mosbahi, K., Walker, D., James, R., Moore, G. R. & Kleanthous, C., Mar 2006, In: Protein science. 15, 3, p. 620-627 8 p.

  Research output: Contribution to journal › Article › peer-review
• 2005

  Cell entry mechanism of enzymatic bacterial colicins: Porin recruitment and the thermodynamics of receptor binding

  Housden, N. G., Loftus, S. R., Moore, G. R., James, R. & Kleanthous, C., 27 Sept 2005, In: Proceedings of the National Academy of Sciences of the United States of America. 102, 39, p. 13849-13854 6 p.

  Research output: Contribution to journal › Article › peer-review

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• Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9

  Tozawa, K., Macdonald, C. J., Penfold, C. N., James, R., Kleanthous, C., Clayden, N. J. & Moore, G. R., 30 Aug 2005, In: Biochemistry. 44, 34, p. 11496-11507 12 p.

  Research output: Contribution to journal › Article › peer-review

• Identification of the metal environment in the stress-regulated antisigma factor RsrA from Streptomyces coelicolor

  Zdanowski, K., Paget, M. & Kleanthous, C., 4 Mar 2005, In: The FASEB Journal. 19, 4, p. A873-A873 1 p.

  Research output: Contribution to journal › Article › peer-review

• Metal induced selectivity in phosphate ion binding in E9 DNase

  van den Bremer, E. T. J., Keeble, A. H., Kleanthous, C. & Heck, A. J. R., 2005, In: Chemical Communications. 9, 9, p. 1137-1139 3 p.

  Research output: Contribution to journal › Article › peer-review

• Rapid detection of colicin E9-induced DNA damage using Escherichia coli cells carrying SOS promoter-lux fusions

  Vankemmelbeke, M., Healy, B., Moore, G. R., Kleanthous, C., Penfold, C. N. & James, R., Jul 2005, In: Journal of Bacteriology. 187, 14, p. 4900-4907 8 p.

  Research output: Contribution to journal › Article › peer-review

• Rapid detection of colicin E9-induced DNA damage using Escherichia coli cells carrying SOS promoter-lux fusions (vol 187, pg 4900, 2005)

  Vankemmelbeke, M., Healy, B., Moore, G. R., Kleanthous, C., Penfold, C. N. & James, R., Sept 2005, In: Journal of Bacteriology. 187, 18, p. 6601-6601 1 p.

  Research output: Contribution to journal › Article › peer-review

• Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry

  van den Heuvel, R. H. H., Gato, S., Versluis, C., Gerbaux, P., Kleanthous, C. & Heck, A. J. R., 2005, In: Nucleic Acids Research. 33, 10, p. e96

  Research output: Contribution to journal › Article › peer-review

• The kinetic basis for dual recognition in colicin endonuclease-immunity protein complexes

  Keeble, A. H. & Kleanthous, C., 23 Sept 2005, In: Journal of Molecular Biology. 352, 3, p. 656-671 16 p.

  Research output: Contribution to journal › Article › peer-review

• The role of an activating peptide in protease-mediated suicide of Escherichia coli K12

  Copeland, N. A. & Kleanthous, C., 2005, In: Journal of Biological Chemistry. 280, 1, p. 112-7 6 p.

  Research output: Contribution to journal › Article › peer-review
• 2004

  Characterisation of a mobile protein-binding epitope in the translocation domain of colicin E9

  Macdonald, C. J., Tozawa, K., Penfold, C. N., James, R., Kleanthous, C., Clayden, N. J. & Moore, G. R., Sept 2004, In: JOURNAL OF BIOMOLECULAR NMR. 30, 1, p. 81-96 16 p.

  Research output: Contribution to journal › Article › peer-review

• Destabilization of the colicin E9 Endonuclease domain by interaction with negatively charged phospholipids: implications for colicin translocation into bacteria

  Mosbahi, K., Walker, D., Lea, E., Moore, G. R., James, R. & Kleanthous, C., 2004, In: Journal of Biological Chemistry. 279, 21, p. 22145-51 7 p.

  Research output: Contribution to journal › Article › peer-review

• Distinct conformational stability and functional activity of four highly homologous endonuclease colicins

  van den Bremer, E. T. J., Keeble, A. H., Jiskoot, W., Spelbrink, R. E. J., Maier, C. S., van Hoek, A., Visser, A. J. W. G., James, R., Moore, G. R., Kleanthous, C. & Heck, A. J. R., May 2004, In: Protein Science. 13, 5, p. 1391-1401 11 p.

  Research output: Contribution to journal › Article › peer-review

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• Flexibility in the receptor-binding domain of the enzymatic colicin E9 is required for toxicity against Escherichia coli cells

  Penfold, C. N., Healy, B., Housden, N. G., Boetzel, R., Vankemmelbeke, M., Kleanthous, C., Moore, G. R. & James, R., Jul 2004, In: Journal of Bacteriology. 186, 14, p. 4520-4527 8 p.

  Research output: Contribution to journal › Article › peer-review

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• Highly discriminating protein-protein interaction specificities in the context of a conserved binding energy hotspot

  Li, W., Keeble, A. H., Giffard, C., James, R., Moore, G. R. & Kleanthous, C., 2004, In: Journal of Molecular Biology. 337, 3, p. 743-59 17 p.

  Research output: Contribution to journal › Article › peer-review

• Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12

  Copeland, N. A., Bingham, R., Georgiou, T., Cooper, P. & Kleanthous, C., 22 Jun 2004, In: Biochemistry. 43, 24, p. 7948-7953 6 p.

  Research output: Contribution to journal › Article › peer-review

• Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3

  WALKER, D., LANCASTER, L., KLEANTHOUS, C. & James, R., Jun 2004, In: Protein Science. 13, 6, p. 1603-1611 9 p.

  Research output: Contribution to journal › Article › peer-review

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