Maggie Smith

Search results

• 2021

  Alanine-scanning mutagenesis of protein mannosyl-transferase from Streptomyces coelicolor reveals strong activity-stability correlation

  Holman, N. D. M., Wilkinson, A. J. & Smith, M. C. M., 22 Oct 2021, In: Microbiology (Reading, England). 167, 10

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2020

  Application of serine integrases for secondary metabolite pathway assembly in Streptomyces

  Gao, H., Taylor, G., Evans, S. K., Fogg, P. C. M. & Smith, M. C. M., 1 Jun 2020, In: Synthetic and Systems Biotechnology. 5, 2, p. 111-119 9 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2019

  Characterisation of the Streptomyces coelicolor glycoproteome reveals glycoproteins important for cell wall biogenesis

  Keenan, T., Dowle, A., Bates, R. & Smith, M. C. M., 25 Jun 2019, In: MBio. 10, 3, 12 p., e01092.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

  Gao, H., Murugesan, B., Hoßbach, J., Evans, S. K., Stark, W. M. & Smith, M. C. M., 4 Jun 2019, In: BMC Biotechnology. 19, 1, 10 p., 32.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Resistance evolution against phage combinations depends on the timing and order of exposure

  Wright, R. C. T., Friman, V-P., Smith, M. C. M. & Brockhurst, M. A., 24 Sept 2019, In: MBio. 10, 5, 15 p., e01652-19.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2018

  Cross-resistance is modular in bacteria-phage interactions

  Wright, R. C. T., Friman, V-P., Smith, M. C. M. & Brockhurst, M. A., 3 Oct 2018, In: PLoS Biology. 16, 10, 22 p., e2006057.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Disruption of the GDP-mannose synthesis pathway in Streptomyces coelicolor results in antibiotic hyper-susceptible phenotypes

  Howlett, R., Anttonen, K., Read, N. & Smith, M. C. M., 1 Apr 2018, In: Microbiology (Reading, England).

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Erratum: Streptomyces coelicolor strains lacking polyprenol phosphate mannose synthase and protein O-mannosyl transferase are hyper-susceptible to multiple antibiotics

  Howlett, R., Read, N., Varghese, A. S., Kershaw, C., Hancock, Y. & Smith, M. C. M., 5 Apr 2018, In: Microbiology (Reading, England). 164, 4, p. 725

  Research output: Contribution to journal › Article › peer-review

• Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif

  Fogg, P. C. M., Younger, E., Fernando, B. D., Khaleel, T., Stark, W. M. & Smith, M. C. M., 16 Feb 2018, In: Nucleic Acids Research. 46, 3, p. 1308-1320 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Streptomyces coelicolor strains lacking polyprenol phosphate mannose synthase and protein O-mannosyl transferase are hyper-susceptible to multiple antibiotics

  Howlett, R., Read, N., Varghese, A. S., Kershaw, C., Hancock, Y. & Smith, M. C. M., 1 Mar 2018, In: Microbiology. 164, p. 369-382 14 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2017

  Control of serine integrase recombination directionality by fusion with the directionality factor

  Olorunniji, F. J., McPherson, A. L., Rosser, S. J., Smith, M. C. M., Colloms, S. D. & Stark, W. M., 21 Aug 2017, In: Nucleic Acids Research. 45, 14, p. 8635-8645 11 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• Genome integration and excision by a new Streptomyces bacteriophage, ϕJoe

  Fogg, P. C. M., Haley, J. A., Stark, W. M. & Smith, M. C. M., 15 Feb 2017, In: Applied and Environmental Microbiology. 83, 5, p. 1-43 43 p., e02767-16.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2016

  Complete genome sequence of 'Mycobacterium neoaurum' NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols

  Rodríguez-García, A., Fernández-Alegre, E., Morales, A., Sola-Landa, A., Lorraine, J., Macdonald, S., Dovbnya, D., Smith, M. C. M., Donova, M. & Barreiro, C., 20 Apr 2016, In: Journal of biotechnology. 224, p. 64-65 2 p.

  Research output: Contribution to journal › Article › peer-review

• The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling

  Pokhilko, A., Zhao, J., Ebenhöh, O., Smith, M. C. M., Stark, W. M. & Colloms, S. D., 6 Sept 2016, In: Nucleic Acids Research. 44, 15, p. 7360-7372 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2015

  Multiplexed integrating plasmids for engineering the erythromycin gene cluster for expression in Streptomyces and combinatorial biosynthesis

  Fayed, B., Ashford, D. A., Hashem, A. M., Amin, M. A., El Gazayerly, O. N., Gregory, M. A. & Smith, M. C. M., Dec 2015, In: Applied and Environmental Microbiology. 81, 23, p. 1-36 36 p.

  Research output: Contribution to journal › Article › peer-review

• Phage-encoded Serine Integrases and Other Large Serine Recombinases

  Smith, M. C. M., 2 Jul 2015, In: Microbiology spectrum. 3, 4, p. 253-272 20 p.

  Research output: Contribution to journal › Article › peer-review

• The phage growth limitation system in Streptomyces coelicolor A(3)2 is a toxin/antitoxin system, comprising enzymes with DNA methyltransferase, protein kinase and ATPase activity

  Hoskisson, P. A., Sumby, P. & Smith, M. C. M., Mar 2015, In: VIROLOGY. 477, p. 100-109 10 p.

  Research output: Contribution to journal › Article › peer-review
• 2014

  An Efficient Method for the In Vitro Production of Azol(in)e-Based Cyclic Peptides

  Houssen, W. E., Bent, A. F., McEwan, A. R., Pieiller, N., Tabudravu, J., Koehnke, J., Mann, G., Adaba, R. I., Thomas, L., Hawas, U. W., Liu, H., Schwarz-Linek, U., Smith, M. C. M., Naismith, J. H. & Jaspars, M., 21 Oct 2014, In: Angewandte Chemie International Edition. 53, 51, p. 14171–14174 4 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

• A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

  Fayed, B., Younger, E., Taylor, G. & Smith, M., 30 May 2014, In: BMC Biotechnology. 14, 7 p., 51.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• New Applications for Phage Integrases

  Fogg, P. C. M., Colloms, S., Rosser, S., Stark, M. & Smith, M. C. M., 29 Jul 2014, In: Journal of Molecular Biology. 426, 15, p. 2703-2716 14 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

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• 2013

  Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

  Xu, Z., Thomas, L., Davies, B., Chalmers, R., Smith, M. & Brown, W., 20 Oct 2013, In: BMC Biotechnology. 13, 87.

  Research output: Contribution to journal › Article › peer-review

• A Flexible Mathematical Model Platform for Studying Branching Networks: Experimentally Validated Using the Model Actinomycete, Streptomyces coelicolor

  Nieminen, L., Webb, S., Smith, M. C. M. & Hoskisson, P. A., 18 Feb 2013, In: PLoS ONE. 8, 2, e54316.

  Research output: Contribution to journal › Article › peer-review

• An enzymatic route to selenazolines

  Koehnke, J., Morawitz, F., Bent, A. F., Houssen, W. E., Shirran, S. L., Fuszard, M. A., Smellie, I. A., Botting, C. H., Smith, M. C. M., Jaspars, M. & Naismith, J. H., 18 Mar 2013, In: Chembiochem. 14, 5, p. 564-567

  Research output: Contribution to journal › Article › peer-review

• Evolutionary Relationships among Actinophages and a Putative Adaptation for Growth in Streptomyces spp

  Smith, M. C. M., Hendrix, R. W., Dedrick, R., Mitchell, K., Ko, C-C., Russell, D., Bell, E., Gregory, M., Bibb, M. J., Pethick, F., Jacobs-Sera, D., Herron, P., Buttner, M. J. & Hatfull, G. F., 30 Aug 2013, In: Journal of Bacteriology.

  Research output: Contribution to journal › Article › peer-review

• Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

  Colloms, S. D., Merrick, C. A., Olorunniji, F. J., Stark, W. M., Smith, M. C. M., Osbourn, A., Keasling, J. D. & Rosser, S. J., 12 Nov 2013, In: Nucleic Acids Research. 42, 4, e23.

  Research output: Contribution to journal › Article › peer-review

  Open Access

• Structure of PatF from Prochloron didemni

  Bent, A. F., Koehnke, J., Houssen, W. E., Smith, M. C. M., Jaspars, M. & Naismith, J. H., Jun 2013, In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 69, 6, p. 618-623 6 p.

  Research output: Contribution to journal › Article › peer-review
• 2012

  Gated rotation mechanism of site-specific recombination by ϕC31 integrase

  Olorunniji, F. J., Buck, D. E., Colloms, S. D., McEwan, A. R., Smith, M. C. M., Stark, W. M. & Rosser, S. J., 27 Nov 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 48, p. 19661-19666 6 p.

  Research output: Contribution to journal › Article › peer-review

• Metabolic Switches and Adaptations Deduced from the Proteomes of Streptomyces coelicolor Wild Type and phoP Mutant Grown in Batch Culture

  Thomas, L., Hodgson, D. A., Wentzel, A., Nieselt, K., Ellingsen, T. E., Moore, J., Morrissey, E. R., Legaie, R., Wohlleben, W., Rodriguez-Garcia, A., Martin, J. F., Burroughs, N. J., Wellington, E. M. H., Smith, M. C. M. & STREAM Consortium, 1 Feb 2012, In: Molecular and Cellular Proteomics. 11, 2, 17 p., M111.013797.

  Research output: Contribution to journal › Article › peer-review

• The discovery of new cyanobactins from cyanothece PCC 7425 defines a new signature for processing of patellamides

  Houssen, W. E., Koehnke, J., Zollman, D., Vendome, J., Raab, A., Smith, M. C. M., Naismith, J. H. & Jaspars, M., Dec 2012, In: Chembiochem. 13, 18, p. 2683-2689 7 p.

  Research output: Contribution to journal › Article › peer-review

• The mechanism of patellamide macrocyclization revealed by the characterization of the PatG macrocyclase domain

  Koehnke, J., Bent, A., Houssen, W. E., Zollman, D., Morawitz, F., Shirran, S., Vendome, J., Nneoyiegbe, A. F., Trembleau, L., Botting, C. H., Smith, M. C. M., Jaspars, M. & Naismith, J. H., Aug 2012, In: Nature Structural & Molecular Biology. 19, 8, p. 767-772 6 p.

  Research output: Contribution to journal › Article › peer-review
• 2011

  A phage protein that binds phi C31 integrase to switch its directionality

  Khaleel, T., Younger, E., McEwan, A. R., Varghese, A. S. & Smith, M. C. M., Jun 2011, In: Molecular Microbiology. 80, 6, p. 1450-1463 14 p.

  Research output: Contribution to journal › Article › peer-review

• Zinc is essential for high-affinity DNA binding and recombinase activity of phi C31 integrase

  McEwan, A. R., Raab, A., Kelly, S. M., Feldmann, J. & Smith, M. C. M., Aug 2011, In: Nucleic Acids Research. 39, 14, p. 6137-6147 11 p.

  Research output: Contribution to journal › Article › peer-review
• 2010

  Site-specific recombination by phi C31 integrase and other large serine recombinases

  Smith, M. C. M., Brown, W. R. A., McEwan, A. R. & Rowley, P. A., Apr 2010, In: Biochemical Society transactions. 38, 2, p. 388-394 7 p.

  Research output: Contribution to journal › Article › peer-review

• The dynamic architecture of the metabolic switch in Streptomyces coelicolor

  Nieselt, K., Battke, F., Herbig, A., Bruheim, P., Wentzel, A., Jakobsen, O. M., Sletta, H., Alam, M. T., Merlo, M. E., Moore, J., Omara, W. A. M., Morrissey, E. R., Juarez-Hermosillo, M. A., Rodriguez-Garcia, A., Nentwich, M., Thomas, L., Iqbal, M., Legaie, R., Gaze, W. H., Challis, G. L., & 15 othersJansen, R. C., Dijkhuizen, L., Rand, D. A., Wild, D. L., Bonin, M., Reuther, J., Wohlleben, W., Smith, M. C. M., Burroughs, N. J., Martin, J. F., Hodgson, D. A., Takano, E., Breitling, R., Ellingsen, T. E. & Wellington, E. M. H., 6 Jan 2010, In: BMC Genomics. 11, 1, p. - 9 p., 10.

  Research output: Contribution to journal › Article › peer-review

• Use of an inducible promoter for antibiotic production in a heterologous host

  Dangel, V., Westrich, L., Smith, M. C. M., Heide, L. & Gust, B., Jun 2010, In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 87, 1, p. 261-269 9 p.

  Research output: Contribution to journal › Article › peer-review
• 2009

  DNA binding and synapsis by the large C-terminal domain of phi C31 integrase

  McEwan, A. R., Rowley, P. A. & Smith, M. C. M., Aug 2009, In: Nucleic Acids Research. 37, 14, p. 4764-4773 10 p.

  Research output: Contribution to journal › Article › peer-review

• Glycosylation of the phosphate binding protein, PstS, in Streptomyces coelicolor by a pathway that resembles protein O-mannosylation in eukaryotes

  Wehmeier, S., Varghese, A. S., Gurcha, S. S., Tissot, B., Panico, M., Hitchen, P., Morris, H. R., Besra, G. S., Dell, A. & Smith, M. C. M., Jan 2009, In: Molecular Microbiology. 71, 2, p. 421-433 13 p.

  Research output: Contribution to journal › Article › peer-review
• 2008

  A motif in the C-terminal domain of phi C31 integrase controls the directionality of recombination

  Rowley, P. A., Smith, M. C. A., Younger, E. & Smith, M. C. M., Jul 2008, In: Nucleic Acids Research. 36, 12, p. 3879-3891 13 p.

  Research output: Contribution to journal › Article › peer-review

• Role of the N-terminal domain of phi C31 integrase in attB-attP synapsis

  Rowley, P. A. & Smith, M. C. M., Oct 2008, In: Journal of Bacteriology. 190, 20, p. 6918-6921 4 p.

  Research output: Contribution to journal › Article › peer-review