Cellular stress and aging damages proteins, ultimately these elements form toxic aggregates and accumulate in the cell. However, during cell division, they are prevented from transmission to the new cell forming. As a survival strategy this unequal distribution allow generate new cells free of toxics. I am using super-resolution microscopy for single molecule detection, smart biosensors, as well as microfluidics approaches to characterized biophysical changes and trafficking dynamics behind this phenomenon. Performed on a library of strains I am developing (S.cerevisiae budding yeast as a model), this characterization will help understand and design a model reflecting cellular dynamics taking place in living cells and in response to cellular stress episodes, a valuable readout to optimize the integration and expression of synthetic circuit.