C2D2 research 1b - Point-of-care, electronic detection of pathogenic bacteria using redox-active biomarkers

Project: Other projectOther internal award

Project Details

Layman's description

Our aim is to establish and evaluate a point-of-care diagnostic to provide rapid, sensitive and specific N. meningitidis screening. The tool will combine microelectronic devices fabricated using the same, low-cost technology developed in the semiconductor industry, with molecular probes targeting N. meningitidis specific markers allowing quantitative and rapid screening in a single, simple readout, portable device. The technology developed through this programme is relevant to the detection of a wide range of pathogenic bacteria and our longer-term vision is to provide a versatile technology to enable rapid diagnosis of bacterial infection at point-of-care.

Key findings

We successfully demonstrated electronic detection of Laz binding to an anti-Laz antibody where the antibody was immobilised covalently onto an electrode surface via a novel alkane/PEG self-assembled monolayer. Detection of Laz – anti-Laz binding was achieved using impedance spectroscopy. We are currently investigating antibody mimetics (peptide aptamers) as alternative binding agents compatible with detection of Laz via measurement of redox potentials. To this end, purified Laz has been expressed and aptamers against Laz are currently being selected.

This project has led directly to collaboration with Dr Alison Parkin (Chemistry, University of York) who has interest in the catalytic activity of redox-active metalloproteins and with Dr Darren Tomlinson (Bioscreening technology group, University of Leeds) for the selection of antibody mimetics (peptide aptamers) against redox-active diagnostic biomarkers.

Approaches developed during the C2D2 project for immobilising biological molecules onto inorganic sensor surfaces has led to a collaboration with Prof. Thomas Krauss (Physics) and Dr Mark Coles (Biology) focussed on photonic biosensors. This work has recently been awarded a BBSRC Tools and Resources Development Fund (details below)



Label-free, Real-time, Spatial-resolution (LRS) immunoassay: 2D mapping of extracellular signalling molecules
PI T. Krauss, Co-I Steve Johnson, Mark Coles
BBSRC Tools and Resources Development Fund (BB/L018160/1)
£181,954.14 (AWARDED)
01/04/2014 - 01/08/2016

James Murray, Dominika Nowak, Laurynas Pukenas, Rizuan Azhar, Mathieu Guillorit, Christoph Wälti, Kevin Critchley, Steven Johnson, and Robin S. Bon,
Solid phase synthesis of functionalised SAM-forming alkanethiol-oligoethyleneglycols
J. Mater. Chem. B DOI: 10.1039/C4TB00573B

Simon J. White1, Steven D. Johnson, Mark A. Sellick, Agnieszka Bronowska, Peter G. Stockley and Christoph Wälti,
The Influence of Two-Dimensional Organization on Peptide Conformation
Angewandte Chemie Int. Ed. DOI: 10.1002/anie.201408971
Effective start/end date1/07/1231/03/13