C2D2 Research 5a - High-throughput flow cytometry for experimental evolutionary microbiology

Flow cytometry is the gold-standard methodology in the field of evolutionary microbiology for estimating microbial fitness and feasibly allows rapid large-scale screening of 1000s of genotypes. To maintain York’s competitiveness in evolutionary microbiology research, and to allow the design of more ambitious and sophisticated experiments, investment is required to establish high-throughput evolutionary analysis of microbes by flow cytometry as an enhancement of the current facilities within the Technology Facility, which are lacking automated sample loading and sufficient capacity. Establishing this method at York will greatly enhance ongoing research projects and generate preliminary data to underpin ambitious future grant applications.