Manipulating lignin to improve biofuel conversion of plant biomass

Project: Research project (funded)Research

Project Details

Key findings

Our component of this project led by the University of Dundee was to develop and implement a high-throughput analytical platform to allow the digestibility of lignocellulosic biomass to be assessed in a sensitive and reliable manner. The platform was successfully established around two automated pieces of equipment, the first to gring straw samples and dispense them accurately into the wells of 96-well plates; and the second a liquid-handling platform to perform pretreatment and enzymatic hydrolysis of these plates, followed by measuring the release of reducing sugars by a colourimetric assay. This platform was eventually established as a robust and reliable tool for screening the very large (10s of thousands) of samples needed for the barley association genetic studies. We have successfully analysed three seasons of barley association genetics panels, allowing our collaborators to identify significant QTL for stem digestibility.
Effective start/end date13/04/0912/04/14


  • BBSRC: £365,687.27