Project Details
Description
£61,220 + £6,200
Key findings
Report on progress, July 2015.
The MST system was installed on 28 July 2014 at which time a number of users were trained.
The second training visit was held in March 2015 which enabled some further new users to be trained by Nanotemper representatives, and also allowed a troubleshooting session for more experienced users. (Future training will be held in house.)
The unfortunate delays in procurement of the system mean that it has been in place less than 12 months, so publications and specific impacts are not yet to be expected, but the system has been used to investigate a number of interactions including plasmid segregation proteins (Barilla group), RNA binding proteins (Plevin, Antson groups), DNA binding proteins (Smith) and quantum dot labels (O’Toole).
Useful expertise has been gained e.g. in the selection of appropriate fluorescent labels for use with the technique, and it has been utilised as proposed to “scout” conditions preparatory to the more elaborate surface plasmon resonance method. Other users are actively considering use of the MST technique for initial screening and also more detailed study of specific interactions.
Take-up by the industrial partner has not so far been as extensive as hoped, but agreements are in place for their personnel to access the system when required.
The MST system was installed on 28 July 2014 at which time a number of users were trained.
The second training visit was held in March 2015 which enabled some further new users to be trained by Nanotemper representatives, and also allowed a troubleshooting session for more experienced users. (Future training will be held in house.)
The unfortunate delays in procurement of the system mean that it has been in place less than 12 months, so publications and specific impacts are not yet to be expected, but the system has been used to investigate a number of interactions including plasmid segregation proteins (Barilla group), RNA binding proteins (Plevin, Antson groups), DNA binding proteins (Smith) and quantum dot labels (O’Toole).
Useful expertise has been gained e.g. in the selection of appropriate fluorescent labels for use with the technique, and it has been utilised as proposed to “scout” conditions preparatory to the more elaborate surface plasmon resonance method. Other users are actively considering use of the MST technique for initial screening and also more detailed study of specific interactions.
Take-up by the industrial partner has not so far been as extensive as hoped, but agreements are in place for their personnel to access the system when required.
| Status | Finished |
|---|---|
| Effective start/end date | 1/08/13 → 31/07/14 |