With the same participants

RPF 2016/17: Anti-microbial resistance and its environmental drivers: A pilot study in wildlife.

Project: Other internal awardInternal pump-priming

Project participant(s)

Department / unit(s)

Description

Amount awarded £9,529

Layman's description

Antimicrobial resistance (AMR) is a major challenge to global health. Currently, we do not understand how AMR moves between humans, livestock and the environment. Wild animals are potential dispersers of AMR. Untreated wastes from humans and livestock dosed with antimicrobial drugs are assumed to be the sources of AMR to wildlife. This pilot project will collect samples from wild mammals and birds on a transect between two anthropogenic drivers of AMR, a farm and a sewage treatment plant. By comparing in detail at ecological, spatial and genetic levels, bacteria in livestock, human and wildlife wastes, we will demonstrate the AMR-dispersal capacity of wildlife.

Key findings

The isolation of antimicrobial resistant bacteria (ARB) from wildlife living adjacent to humans has led to the suggestion that such antimicrobial resistance (AMR) is anthropogenically driven by exposure to antimicrobials and ARB. However, ARB have also been detected in wildlife living in areas without interaction with humans. Here, we investigated patterns of resistance in Escherichia coli isolated from 408 wild bird and mammal faecal samples. AMR and multi-drug resistance (MDR) prevalence in wildlife samples differed significantly between a Sewage Treatment Plant (STP; wastes of antibiotic-treated humans) and a Farm site (antibiotic-treated livestock wastes) and Central site (no sources of wastes containing anthropogenic AMR or antimicrobials), but patterns of resistance also varied significantly over time and between mammals and birds. Over 30% of AMR isolates were resistant to colistin, a last-resort antibiotic, but resistance was not due to the mcr-1 gene. ESBL and AmpC activity were common in isolates from mammals. Wildlife were, therefore, harbouring resistance of clinical relevance. AMR E. coli, including MDR, were found in diverse wildlife species, and the patterns and prevalence of resistance were not consistently associated with site and therefore different exposure risks. We conclude that AMR in commensal bacteria of wildlife is not driven simply by anthropogenic factors, and, in practical terms, this may limit the utility of wildlife as sentinels of spatial variation in the transmission of environmental AMR.
StatusFinished
Effective start/end date1/08/1631/07/17

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