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+ dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins

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Author(s)

  • Meera Parthasarathy
  • Joyashish Debgupta
  • Bhalchandra Kakade
  • Abu A. Ansary
  • M. Islam Khan
  • Vijayamohanan K. Pillai

Department/unit(s)

Publication details

JournalANALYTICAL BIOCHEMISTRY
DatePublished - 15 Feb 2011
Issue number2
Volume409
Number of pages6
Pages (from-to)230-235
Original languageEnglish

Abstract

The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins.

    Research areas

  • Carbon nanotubes, Gel electrophoresis, Molecular weight determination, Polyacrylamide, Protein separation

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