By the same authors

From the same journal

From the same journal

12-Oxo-Phytodienoic Acid Accumulation during Seed Development Represses Seed Germination in Arabidopsis

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalThe Plant Cell
DatePublished - Feb 2011
Issue number2
Volume23
Number of pages17
Pages (from-to)583-599
Original languageEnglish

Abstract

Arabidopsis thaliana COMATOSE (CTS) encodes an ABC transporter involved in peroxisomal import of substrates for beta-oxidation. Various cts alleles and mutants disrupted in steps of peroxisomal beta-oxidation have previously been reported to exhibit a severe block on seed germination. Oxylipin analysis on cts, acyl CoA oxidase1 acyl CoA oxidase2 (acx1 acx2), and keto acyl thiolase2 dry seeds revealed that they contain elevated levels of 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA), and JA-Ile. Oxylipin and transcriptomic analysis showed that accumulation of these oxylipins occurs during late seed maturation in cts. Analysis of double mutants generated by crossing cts with mutants in the JA biosynthesis pathway indicate that OPDA, rather than JA or JA-Ile, contributes to the block on germination in cts seeds. We found that OPDA was more effective at inhibiting wild-type germination than was JA and that this effect was independent of CORONATINE INSENSITIVE1 but was synergistic with abscisic acid (ABA). Consistent with this, OPDA treatment increased ABA INSENSITIVE5 protein abundance in a manner that parallels the inhibitory effect of OPDA and OPDA+ABA on seed germination. These results demonstrate that OPDA acts along with ABA to regulate seed germination in Arabidopsis.

    Research areas

  • BINDING CASSETTE TRANSPORTER, OXO-PHYTODIENOIC ACID, TENDRIL-COILING RESPONSE, ALLENE OXIDE CYCLASE, GENOME-WIDE ANALYSIS, JASMONIC ACID, BETA-OXIDATION, ABSCISIC-ACID, FUNCTIONAL DIVERSIFICATION, CONTAINING GALACTOLIPIDS

Projects

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations