3-DIMENSIONAL STRUCTURE OF NEUROTOXIN-1 FROM NAJA NAJA OXIANA VENOM AT 1.9 Å RESOLUTION

A V  NICKITENKO; A M  MICHAILOV; C  BETZEL; K S  WILSON

3-DIMENSIONAL STRUCTURE OF NEUROTOXIN-1 FROM NAJA NAJA OXIANA VENOM AT 1.9 Å RESOLUTION

A V NICKITENKO, A M MICHAILOV, C BETZEL, K S WILSON

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 angstrom, b = 75.6 angstrom, c = 35.9 angstrom, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 angstrom have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 angstrom resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.

Original language	English
Pages (from-to)	111-117
Number of pages	7
Journal	FEBS Letters
Volume	320
Issue number	2
Publication status	Published - 5 Apr 1993

Cite this

@article{58cacd87801c4965abb1ff28805fd581,

title = "3-DIMENSIONAL STRUCTURE OF NEUROTOXIN-1 FROM NAJA NAJA OXIANA VENOM AT 1.9 {\AA} RESOLUTION",

abstract = "Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 angstrom, b = 75.6 angstrom, c = 35.9 angstrom, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 angstrom have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 angstrom resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.",

author = "NICKITENKO, {A V} and MICHAILOV, {A M} and C BETZEL and WILSON, {K S}",

year = "1993",

month = apr,

day = "5",

language = "English",

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pages = "111--117",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

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T1 - 3-DIMENSIONAL STRUCTURE OF NEUROTOXIN-1 FROM NAJA NAJA OXIANA VENOM AT 1.9 Å RESOLUTION

AU - NICKITENKO, A V

AU - MICHAILOV, A M

AU - BETZEL, C

AU - WILSON, K S

PY - 1993/4/5

Y1 - 1993/4/5

N2 - Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 angstrom, b = 75.6 angstrom, c = 35.9 angstrom, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 angstrom have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 angstrom resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.

AB - Neurotoxin-1 from Naja naja oxiana venom (NTX-1) has been crystallized by vapor diffusion in sitting drops. The crystals have cell dimensions of a = 25.2 angstrom, b = 75.6 angstrom, c = 35.9 angstrom, and are in space group P2(1)2(1)2(1). Three-dimensional data to 1.9 angstrom have been recorded by a Syntex P2(1) automatic diffractometer. The atomic structure of the toxin has been determined by molecular replacement using the alpha-cobratoxin (alpha-CTX) as the search model. The position of 534 non-hydrogen protein atoms have been determined. The model contains 65 water molecules. Refinement has led to an R-factor of 19.3% at 1.9 angstrom resolution. The secondary and tertiary structures of NTX-1 have been analyzed and a comparison with structure of the alpha-CTX has been made.

M3 - Article

SN - 0014-5793

VL - 320

SP - 111

EP - 117

JO - FEBS Letters

JF - FEBS Letters

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