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A ‘catch-and-release’ receptor for the cholera toxin

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Clare Sarah Mahon
  • Gemma C. Wildsmith
  • Diksha Haksar
  • Eyleen de Poel
  • Jeffrey M. Beekman
  • Roland J. Pieters
  • Michael E. Webb
  • W. Bruce Turnbull

Department/unit(s)

Publication details

JournalFARADAY DISCUSSIONS
DateAccepted/In press - 20 Mar 2019
DatePublished (current) - 21 Mar 2019
Number of pages8
Original languageEnglish

Abstract

Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

Bibliographical note

© The Royal Society of Chemistry 2019. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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