A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase

Matthew G. Alteen; Christina Gros; Richard W. Meek; David A. Cardoso; Jil A. Busmann; Gontran Sangouard; Matthew C. Deen; Hong-Yee Tan; David L. Shen; Cecilia C. Russell; Gideon J. Davies; Phillip J. Robinson; Adam McCluskey; David J. Vocadlo

doi:10.1002/ange.202000621

A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase

Matthew G. Alteen, Christina Gros, Richard W. Meek, David A. Cardoso, Jil A. Busmann, Gontran Sangouard, Matthew C. Deen, Hong-Yee Tan, David L. Shen, Cecilia C. Russell, Gideon J. Davies, Phillip J. Robinson, Adam McCluskey, David J. Vocadlo

Chemistry

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	9601-9609
Journal	Angewandte Chemie International Edition
Volume	59
Issue number	24
Early online date	2 Apr 2020
DOIs	https://doi.org/10.1002/ange.202000621 https://doi.org/10.1002/anie.202000621
Publication status	Published - 8 Jun 2020

Bibliographical note

© 2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

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Accepted_Article_Alteen_Manuscript_Feb22
© 2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
Accepted author manuscript, 2.56 MB

RS Research Professorship
Davies, G. J. (Principal investigator)
THE ROYAL SOCIETY
1/01/17 → 31/12/27
Project: Research project (funded) › Research

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Alteen, M. G., Gros, C., Meek, R. W., Cardoso, D. A., Busmann, J. A., Sangouard, G., Deen, M. C., Tan, H.-Y., Shen, D. L., Russell, C. C., Davies, G. J., Robinson, P. J., McCluskey, A., & Vocadlo, D. J. (2020). A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase. Angewandte Chemie International Edition, 59(24), 9601-9609. https://doi.org/10.1002/ange.202000621, https://doi.org/10.1002/anie.202000621

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title = "A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase",

author = "Alteen, {Matthew G.} and Christina Gros and Meek, {Richard W.} and Cardoso, {David A.} and Busmann, {Jil A.} and Gontran Sangouard and Deen, {Matthew C.} and Hong-Yee Tan and Shen, {David L.} and Russell, {Cecilia C.} and Davies, {Gideon J.} and Robinson, {Phillip J.} and Adam McCluskey and Vocadlo, {David J.}",

note = "{\textcopyright} 2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher{\textquoteright}s self-archiving policy. Further copying may not be permitted; contact the publisher for details.",

year = "2020",

month = jun,

day = "8",

doi = "10.1002/ange.202000621",

language = "English",

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Alteen, MG, Gros, C, Meek, RW, Cardoso, DA, Busmann, JA, Sangouard, G, Deen, MC, Tan, H-Y, Shen, DL, Russell, CC, Davies, GJ, Robinson, PJ, McCluskey, A & Vocadlo, DJ 2020, 'A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase', Angewandte Chemie International Edition, vol. 59, no. 24, pp. 9601-9609. https://doi.org/10.1002/ange.202000621, https://doi.org/10.1002/anie.202000621

TY - JOUR

T1 - A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening

T2 - Application to O‐GlcNAc Transferase

AU - Alteen, Matthew G.

AU - Gros, Christina

AU - Meek, Richard W.

AU - Cardoso, David A.

AU - Busmann, Jil A.

AU - Sangouard, Gontran

AU - Deen, Matthew C.

AU - Tan, Hong-Yee

AU - Shen, David L.

AU - Russell, Cecilia C.

AU - Davies, Gideon J.

AU - Robinson, Phillip J.

AU - McCluskey, Adam

AU - Vocadlo, David J.

N1 - © 2020 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2020/6/8

Y1 - 2020/6/8

U2 - 10.1002/ange.202000621

DO - 10.1002/ange.202000621

M3 - Article

SN - 1433-7851

VL - 59

SP - 9601

EP - 9609

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 24

ER -

A Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High‐Throughput Screening: Application to O‐GlcNAc Transferase

Bibliographical note

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Projects

RS Research Professorship

Cite this