A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula

TY - JOUR

T1 - A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula

AU - Wang, Ertao

AU - Yu, Nan

AU - Bano, S. Asma

AU - Liu, Chengwu

AU - Miller, Anthony J.

AU - Cousins, Donna

AU - Zhang, Xiaowei

AU - Ratet, Pascal

AU - Tadege, Million

AU - Mysore, Kirankumar S.

AU - Downie, J. Allan

AU - Murray, Jeremy D.

AU - Oldroyd, Giles E. D.

AU - Schultze, Michael

N1 - © 2014 American Society of Plant Biologists. All rights reserved.

PY - 2014/4/29

Y1 - 2014/4/29

N2 - Most plant species form symbioses with arbuscular mycorrhizal (AM) fungi, which facilitate the uptake of mineral nutrients such as phosphate from the soil. Several transporters, particularly proton-coupled phosphate transporters, have been identified on both the plant and fungal membranes and contribute to delivering phosphate from fungi to plants. The mechanism of nutrient exchange has been studied in plants during mycorrhizal colonization, but the source of the electrochemical proton gradient that drives nutrient exchange is not known. Here, we show that plasma membrane H(+)-ATPases that are specifically induced in arbuscule-containing cells are required for enhanced proton pumping activity in membrane vesicles from AM-colonized roots of rice (Oryza sativa) and Medicago truncatula. Mutation of the H(+)-ATPases reduced arbuscule size and impaired nutrient uptake by the host plant through the mycorrhizal symbiosis. Overexpression of the H(+)-ATPase Os-HA1 increased both phosphate uptake and the plasma membrane potential, suggesting that this H(+)-ATPase plays a key role in energizing the periarbuscular membrane, thereby facilitating nutrient exchange in arbusculated plant cells.

AB - Most plant species form symbioses with arbuscular mycorrhizal (AM) fungi, which facilitate the uptake of mineral nutrients such as phosphate from the soil. Several transporters, particularly proton-coupled phosphate transporters, have been identified on both the plant and fungal membranes and contribute to delivering phosphate from fungi to plants. The mechanism of nutrient exchange has been studied in plants during mycorrhizal colonization, but the source of the electrochemical proton gradient that drives nutrient exchange is not known. Here, we show that plasma membrane H(+)-ATPases that are specifically induced in arbuscule-containing cells are required for enhanced proton pumping activity in membrane vesicles from AM-colonized roots of rice (Oryza sativa) and Medicago truncatula. Mutation of the H(+)-ATPases reduced arbuscule size and impaired nutrient uptake by the host plant through the mycorrhizal symbiosis. Overexpression of the H(+)-ATPase Os-HA1 increased both phosphate uptake and the plasma membrane potential, suggesting that this H(+)-ATPase plays a key role in energizing the periarbuscular membrane, thereby facilitating nutrient exchange in arbusculated plant cells.

U2 - 10.1105/tpc.113.120527

DO - 10.1105/tpc.113.120527

M3 - Article

C2 - 24781115

SN - 1040-4651

VL - 26

SP - 1818

EP - 1830

JO - The Plant Cell

JF - The Plant Cell

IS - 4

ER -