A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula

Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E. D. Oldroyd, Michael Schultze

Research output: Contribution to journalArticlepeer-review


Most plant species form symbioses with arbuscular mycorrhizal (AM) fungi, which facilitate the uptake of mineral nutrients such as phosphate from the soil. Several transporters, particularly proton-coupled phosphate transporters, have been identified on both the plant and fungal membranes and contribute to delivering phosphate from fungi to plants. The mechanism of nutrient exchange has been studied in plants during mycorrhizal colonization, but the source of the electrochemical proton gradient that drives nutrient exchange is not known. Here, we show that plasma membrane H(+)-ATPases that are specifically induced in arbuscule-containing cells are required for enhanced proton pumping activity in membrane vesicles from AM-colonized roots of rice (Oryza sativa) and Medicago truncatula. Mutation of the H(+)-ATPases reduced arbuscule size and impaired nutrient uptake by the host plant through the mycorrhizal symbiosis. Overexpression of the H(+)-ATPase Os-HA1 increased both phosphate uptake and the plasma membrane potential, suggesting that this H(+)-ATPase plays a key role in energizing the periarbuscular membrane, thereby facilitating nutrient exchange in arbusculated plant cells.

Original languageEnglish
Pages (from-to)1818-1830
Number of pages13
JournalThe Plant Cell
Issue number4
Publication statusPublished - 29 Apr 2014

Bibliographical note

© 2014 American Society of Plant Biologists. All rights reserved.

Cite this