A high-throughput colourimetric method for the determination of pH in crystallization screens

Jobie Kirkwood*, Julie Wilson, Simon O'keefe, David Hargreaves

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The crystallization of proteins is dependent on the careful control of numerous parameters, one of these being pH. The pH of crystallization is generally reported as that of the buffer; however, the true pH has been found to be as many as four pH units away. Measurement of pH with a meter is time-consuming and requires the reformatting of the crystallization solution. To overcome this, a high-throughput method for pH determination of buffered solutions has been developed with results comparable to those of a pH meter.

Original languageEnglish
Pages (from-to)2367-2375
Number of pages9
JournalActa Crystallographica. Section D, Biological Crystallography
Volume70
Issue number9
DOIs
Publication statusPublished - 1 Jan 2014

Bibliographical note

© 2014 International Union of Crystallography. Reproduced in accordance with the publisher's self-archiving policy.

Keywords

  • absorbance
  • bromothymol blue
  • colourimetry
  • crystallization
  • indicator dye
  • pH
  • spectrophotometry

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