A local regulatory network around three NAC transcription factors in stress responses and senescence in Arabidopsis leaves

Richard Hickman, Claire Hill, Christopher A. Penfold, Emily Breeze, Laura Bowden, Jonathan D. Moore, Peijun Zhang, Alison Jackson, Emma Cooke, Findlay Bewicke-Copley, Andrew Mead, Jim Beynon, David L. Wild, Katherine J. Denby, Sascha Ott, Vicky Buchanan-Wollaston*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A model is presented describing the gene regulatory network surrounding three similar NAC transcription factors that have roles in Arabidopsis leaf senescence and stress responses. ANAC019, ANAC055 and ANAC072 belong to the same clade of NAC domain genes and have overlapping expression patterns. A combination of promoter DNA/protein interactions identified using yeast 1-hybrid analysis and modelling using gene expression time course data has been applied to predict the regulatory network upstream of these genes. Similarities and divergence in regulation during a variety of stress responses are predicted by different combinations of upstream transcription factors binding and also by the modelling. Mutant analysis with potential upstream genes was used to test and confirm some of the predicted interactions. Gene expression analysis in mutants of ANAC019 and ANAC055 at different times during leaf senescence has revealed a distinctly different role for each of these genes. Yeast 1-hybrid analysis is shown to be a valuable tool that can distinguish clades of binding proteins and be used to test and quantify protein binding to predicted promoter motifs.

Original languageEnglish
Pages (from-to)26-39
Number of pages14
JournalPlant Journal
Volume75
Issue number1
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Arabidopsis thaliana
  • Botrytis cinerea
  • gene regulatory network
  • NAC transcription factors
  • senescence
  • stress

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