By the same authors

From the same journal

A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NolL in Rhizobium leguminosarum

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalMOLECULAR PLANT-MICROBE INTERACTIONS
DatePublished - Apr 2000
Issue number4
Volume13
Number of pages5
Pages (from-to)475-479
Original languageEnglish

Abstract

Heterologous expression of NodZ and NoIL proteins in Rhizobium leguminosarum by. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NoIL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NoIL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NoIL protein caused elevated production of LCOs, Efficient nodulation of Lotus japonicus by the NodZ/NoIL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).

    Research areas

  • acetyl fucose, mass spectrometry, Nod factors, STRUCTURAL IDENTIFICATION, HOST-RANGE, PLASMID, SIGNALS, GENES, OLIGOSACCHARIDES, SYMBIOSIS, INDUCTION, PROTEINS, PLANTS

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations