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A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme

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A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme. / Evans, G ; Wilson, K S .

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, 01.1999, p. 67-76.

Research output: Contribution to journalArticle

Harvard

Evans, G & Wilson, KS 1999, 'A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme', Acta Crystallographica Section D: Biological Crystallography, vol. 55, pp. 67-76.

APA

Evans, G., & Wilson, K. S. (1999). A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme. Acta Crystallographica Section D: Biological Crystallography, 55, 67-76.

Vancouver

Evans G, Wilson KS. A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme. Acta Crystallographica Section D: Biological Crystallography. 1999 Jan;55:67-76.

Author

Evans, G ; Wilson, K S . / A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme. In: Acta Crystallographica Section D: Biological Crystallography. 1999 ; Vol. 55. pp. 67-76.

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@article{f0ad603332fe43f09d0effde13b659a1,
title = "A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme",
abstract = "A multiple-wavelength anomalous diffraction (MAD) experiment was performed on an iridium derivative of hen egg-white lysozyme, Diffraction data were measured at five wavelengths on the X31 EMBL beamline on the DORIS III storage ring and at two further wavelengths on the X11 beamline, Four iridium-binding sites were located from the dispersive anomalous differences between two wavelengths at the rising and falling inflection points of the Ir L-III-edge white line using direct methods. All other attempts to determine the heavy-atom positions failed. The results demonstrate an experimental method whereby systematic error in MAD data due to sample absorption can be reduced where a white line is present in the absorption spectrum of a heavy atom.",
author = "G Evans and Wilson, {K S}",
year = "1999",
month = "1",
language = "English",
volume = "55",
pages = "67--76",
journal = "Acta Crystallographica Section D: Biological Crystallography",
issn = "0907-4449",
publisher = "International Union of Crystallography",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A MAD experiment performed at the white line of the iridium LIII absorption edge in lysozyme

AU - Evans, G

AU - Wilson, K S

PY - 1999/1

Y1 - 1999/1

N2 - A multiple-wavelength anomalous diffraction (MAD) experiment was performed on an iridium derivative of hen egg-white lysozyme, Diffraction data were measured at five wavelengths on the X31 EMBL beamline on the DORIS III storage ring and at two further wavelengths on the X11 beamline, Four iridium-binding sites were located from the dispersive anomalous differences between two wavelengths at the rising and falling inflection points of the Ir L-III-edge white line using direct methods. All other attempts to determine the heavy-atom positions failed. The results demonstrate an experimental method whereby systematic error in MAD data due to sample absorption can be reduced where a white line is present in the absorption spectrum of a heavy atom.

AB - A multiple-wavelength anomalous diffraction (MAD) experiment was performed on an iridium derivative of hen egg-white lysozyme, Diffraction data were measured at five wavelengths on the X31 EMBL beamline on the DORIS III storage ring and at two further wavelengths on the X11 beamline, Four iridium-binding sites were located from the dispersive anomalous differences between two wavelengths at the rising and falling inflection points of the Ir L-III-edge white line using direct methods. All other attempts to determine the heavy-atom positions failed. The results demonstrate an experimental method whereby systematic error in MAD data due to sample absorption can be reduced where a white line is present in the absorption spectrum of a heavy atom.

M3 - Article

VL - 55

SP - 67

EP - 76

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

SN - 0907-4449

ER -