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A new mutation in spo0A with intragenic suppressors in the effector domain

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JournalFEMS microbiology letters
DatePublished - 15 Apr 2000
Issue number2
Volume185
Number of pages6
Pages (from-to)123-128
Original languageEnglish

Abstract

Spo0A is a two domain response regulator, a key protein in the initiation of sporulation of Bacillus subtilis. This protein controls a number of changes in gene expression that occur during the transition from stationary phase to the onset of sporulation. The phosphorylated form of Spo0A influences the transcription of a specific set of genes. In addition to others, it represses abrB and activates spolIIA and spoIIE transcription. Although the N-terminal phosphoacceptor domain is well characterised, there is Limited information on the C-terminal, DNA-binding domain. Comparisons of Spo0A homologues from a number of Bacillus and Clostridium species show that the C-terminal domain contains three highly conserved regions. In this study, we have investigated the influence of spo0A mutations mapping within the C-terminal domain on transcription from the abrB, spoIIA and spoIIE promoters using lacZ fusions. Our results indicate that described mutations can be part of signalling between N- and C-terminal domains of the protein. Also, the increased expression observed from the spoIIE promoter in some Spo0A mutants might result from a stabilising function of these mutations on the transcriptional apparatus utilising sigma(A). (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

    Research areas

  • sporulation, mutagenesis, suppressor mutation, beta-galactosidase assay, sigma factor, Bacillus subtilis, BACILLUS-SUBTILIS, TRANSCRIPTION FACTOR, PROMOTER, SPORULATION, REGION, GENE, CONSTRUCTION, ACTIVATION, PROTEIN, SITE

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