A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system

B M Wilkinson; L Morris; D J Adams; E G Evans; C J Lacey; R M Walmsley

A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system

B M Wilkinson, L Morris, D J Adams, E G Evans, C J Lacey, R M Walmsley

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

The repetitive DNA sequence poly[d(GT).d(CA)],(polyGT) can be used to generate DNA fingerprints that distinguish different yeast genera. In this study we demonstrate that the probe can also be used to distinguish individual strains of clinical isolates of Candida albicans. Isolates were fingerprinted by probing Southern blots of restriction enzyme-cleaved DNA samples with radioactively labelled polyGT. The discrimination between strains was clearer than can be achieved by direct visualization of ethidium bromide stained gels and was comparable to that achieved with previous DNA probes. However, the advantage of this probe is that it is not limited to Candida species since polyGT sequences appear to be ubiquitous in eukaryotes. Fingerprints were also generated from Southern blots using a commercial (AMBIS) radioanalytical imaging computer system. A proprietary software package (MICRO PM) was effective in discriminating between the C. albicans strains. These preliminary results indicate the potential value of this probe for discrimination between Candida isolates in epidemiological studies of candidosis.

Original language	English
Pages (from-to)	123-31
Number of pages	9
Journal	Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
Volume	30
Issue number	2
Publication status	Published - 1992

Keywords

Blotting, Southern
Candida albicans
Cluster Analysis
DNA Fingerprinting
DNA Probes
DNA, Fungal
Female
Humans
Image Processing, Computer-Assisted
Polydeoxyribonucleotides
Repetitive Sequences, Nucleic Acid
Reproducibility of Results
Software
Vagina

Cite this

Wilkinson, B. M., Morris, L., Adams, D. J., Evans, E. G., Lacey, C. J., & Walmsley, R. M. (1992). A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system. Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, 30(2), 123-31.

Wilkinson, B M ; Morris, L ; Adams, D J et al. / A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system. In: Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology. 1992 ; Vol. 30, No. 2. pp. 123-31.

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title = "A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system",

abstract = "The repetitive DNA sequence poly[d(GT).d(CA)],(polyGT) can be used to generate DNA fingerprints that distinguish different yeast genera. In this study we demonstrate that the probe can also be used to distinguish individual strains of clinical isolates of Candida albicans. Isolates were fingerprinted by probing Southern blots of restriction enzyme-cleaved DNA samples with radioactively labelled polyGT. The discrimination between strains was clearer than can be achieved by direct visualization of ethidium bromide stained gels and was comparable to that achieved with previous DNA probes. However, the advantage of this probe is that it is not limited to Candida species since polyGT sequences appear to be ubiquitous in eukaryotes. Fingerprints were also generated from Southern blots using a commercial (AMBIS) radioanalytical imaging computer system. A proprietary software package (MICRO PM) was effective in discriminating between the C. albicans strains. These preliminary results indicate the potential value of this probe for discrimination between Candida isolates in epidemiological studies of candidosis.",

keywords = "Blotting, Southern, Candida albicans, Cluster Analysis, DNA Fingerprinting, DNA Probes, DNA, Fungal, Female, Humans, Image Processing, Computer-Assisted, Polydeoxyribonucleotides, Repetitive Sequences, Nucleic Acid, Reproducibility of Results, Software, Vagina",

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year = "1992",

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Wilkinson, BM, Morris, L, Adams, DJ, Evans, EG, Lacey, CJ & Walmsley, RM 1992, 'A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system', Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, vol. 30, no. 2, pp. 123-31.

A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system. / Wilkinson, B M; Morris, L; Adams, D J et al.
In: Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, Vol. 30, No. 2, 1992, p. 123-31.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system

AU - Wilkinson, B M

AU - Morris, L

AU - Adams, D J

AU - Evans, E G

AU - Lacey, C J

AU - Walmsley, R M

PY - 1992

Y1 - 1992

N2 - The repetitive DNA sequence poly[d(GT).d(CA)],(polyGT) can be used to generate DNA fingerprints that distinguish different yeast genera. In this study we demonstrate that the probe can also be used to distinguish individual strains of clinical isolates of Candida albicans. Isolates were fingerprinted by probing Southern blots of restriction enzyme-cleaved DNA samples with radioactively labelled polyGT. The discrimination between strains was clearer than can be achieved by direct visualization of ethidium bromide stained gels and was comparable to that achieved with previous DNA probes. However, the advantage of this probe is that it is not limited to Candida species since polyGT sequences appear to be ubiquitous in eukaryotes. Fingerprints were also generated from Southern blots using a commercial (AMBIS) radioanalytical imaging computer system. A proprietary software package (MICRO PM) was effective in discriminating between the C. albicans strains. These preliminary results indicate the potential value of this probe for discrimination between Candida isolates in epidemiological studies of candidosis.

AB - The repetitive DNA sequence poly[d(GT).d(CA)],(polyGT) can be used to generate DNA fingerprints that distinguish different yeast genera. In this study we demonstrate that the probe can also be used to distinguish individual strains of clinical isolates of Candida albicans. Isolates were fingerprinted by probing Southern blots of restriction enzyme-cleaved DNA samples with radioactively labelled polyGT. The discrimination between strains was clearer than can be achieved by direct visualization of ethidium bromide stained gels and was comparable to that achieved with previous DNA probes. However, the advantage of this probe is that it is not limited to Candida species since polyGT sequences appear to be ubiquitous in eukaryotes. Fingerprints were also generated from Southern blots using a commercial (AMBIS) radioanalytical imaging computer system. A proprietary software package (MICRO PM) was effective in discriminating between the C. albicans strains. These preliminary results indicate the potential value of this probe for discrimination between Candida isolates in epidemiological studies of candidosis.

KW - Blotting, Southern

KW - Candida albicans

KW - Cluster Analysis

KW - DNA Fingerprinting

KW - DNA Probes

KW - DNA, Fungal

KW - Female

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Polydeoxyribonucleotides

KW - Repetitive Sequences, Nucleic Acid

KW - Reproducibility of Results

KW - Software

KW - Vagina

M3 - Article

C2 - 1588462

SN - 0268-1218

VL - 30

SP - 123

EP - 131

JO - Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology

JF - Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology

IS - 2

ER -

Wilkinson BM, Morris L, Adams DJ, Evans EG, Lacey CJ, Walmsley RM. A new, sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern comparison system. Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology. 1992;30(2):123-31.