A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

Bahgat Fayed, Ellen Younger, Gabrielle Taylor, Maggie Smith

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely,
particularly for cloning genes in Streptomyces spp.
Results: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP
site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails
to generate stable integrants in S. venezuelae, the natural host for phage SV1.
Conclusion: pBF3 promises to be a useful addition to the range of integrating vectors currently available for
Streptomyces molecular genetics.
Original languageEnglish
Article number51
Number of pages7
JournalBMC Biotechnology
Volume14
DOIs
Publication statusPublished - 30 May 2014

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Keywords

  • Streptomyces
  • Cloning
  • Integration vector
  • Serine integrase
  • Bacteriophage
  • SV1

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  • A novel Streptomyces spp integration vector derived from the S. venezuelae phage, SV1

    This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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