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A POLYMERASE CHAIN-REACTION (PCR) INVESTIGATION OF ORAL VERRUCAE WHICH CONTAIN HPV TYPE-2 AND TYPE-57 BY IN-SITU HYBRIDIZATION

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Publication details

JournalJournal of oral pathology & medicine
DatePublished - Aug 1995
Issue number7
Volume24
Number of pages6
Pages (from-to)329-334
Original languageEnglish

Abstract

The polymerase chain reaction (PCR) and direct DNA sequencing have been used to identify strain variants of HPV types 2a/57 in formalin-fixed sections of human oral verrucae, where the virus had previously been detected by both immunofluorescence and in situ hybridization. By employing type-specific and type-common PCR primers we show that these lesions contain a mixture of viral DNAs which vary by up to 27% in DNA sequence, in a region where the variation between HPV types 2a and 57 is only 4%. The extra discriminatory power of fluorescent sequencing indicates that the lesions may also contain wildtype HPV2a/57 DNA which could provide a helper function for defective viral DNA molecules or indicate a mosaic origin for the lesions.

    Research areas

  • HPV 2(X)/57(C) SUBTYPE, HUMAN PAPILLOMAVIRUS, ORAL VERRUCAE, PCR, HUMAN PAPILLOMAVIRUS TYPES, INSITU DNA HYBRIDIZATION, MUCOSAL LESIONS, WARTS, IDENTIFICATION, INFECTION, SEQUENCES, VULGARIS, CAVITY, TISSUE

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