A role for the root cap in root branching revealed by the non-auxin probe naxillin.

Bert De Rybel; Dominique Audenaert; Wei Xuan; Paul Overvoorde; Lucia C  Strader; S. Kepinski; Rebecca Hoye; Ronald Brisbois; Boris Parizot; Steffen Vanneste; Xing Liu; Alison Gilday; Ian Alexander Graham; Long Nguyen; Leentje Jansen; Maria Fransisca Njo; Dirk Inze; Bonnie Bartel; Tom Beeckman

doi:10.1038/nchembio.1044

A role for the root cap in root branching revealed by the non-auxin probe naxillin.

Bert De Rybel, Dominique Audenaert, Wei Xuan, Paul Overvoorde, Lucia C Strader, S. Kepinski, Rebecca Hoye, Ronald Brisbois, Boris Parizot, Steffen Vanneste, Xing Liu, Alison Gilday, Ian Alexander Graham, Long Nguyen, Leentje Jansen, Maria Fransisca Njo, Dirk Inze, Bonnie Bartel, Tom Beeckman

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

he acquisition of water and nutrients by plant roots is a fundamental aspect of agriculture and strongly depends on root architecture. Root branching and expansion of the root system is achieved through the development of lateral roots and is to a large extent controlled by the plant hormone auxin. However, the pleiotropic effects of auxin or auxin-like molecules on root systems complicate the study of lateral root development. Here we describe a small-molecule screen in Arabidopsis thaliana that identified naxillin as what is to our knowledge the first non-auxin-like molecule that promotes root branching. By using naxillin as a chemical tool, we identified a new function for root cap–specific conversion of the auxin precursor indole-3-butyric acid into the active auxin indole-3-acetic acid and uncovered the involvement of the root cap in root branching. Delivery of an auxin precursor in peripheral tissues such as the root cap might represent an important mechanism shaping root architecture.

Original language	English
Pages (from-to)	798-805
Number of pages	8
Journal	NATURE CHEMICAL BIOLOGY
Volume	8
Issue number	9
Early online date	12 Aug 2012
DOIs	https://doi.org/10.1038/nchembio.1044
Publication status	Published - Sept 2012

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10.1038/nchembio.1044

Cite this

De Rybel, B., Audenaert, D., Xuan, W., Overvoorde, P., Strader, L. C., Kepinski, S., Hoye, R., Brisbois, R., Parizot, B., Vanneste, S., Liu, X., Gilday, A., Graham, I. A., Nguyen, L., Jansen, L., Njo, M. F., Inze, D., Bartel, B., & Beeckman, T. (2012). A role for the root cap in root branching revealed by the non-auxin probe naxillin. NATURE CHEMICAL BIOLOGY, 8(9), 798-805. https://doi.org/10.1038/nchembio.1044

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title = "A role for the root cap in root branching revealed by the non-auxin probe naxillin.",

abstract = "he acquisition of water and nutrients by plant roots is a fundamental aspect of agriculture and strongly depends on root architecture. Root branching and expansion of the root system is achieved through the development of lateral roots and is to a large extent controlled by the plant hormone auxin. However, the pleiotropic effects of auxin or auxin-like molecules on root systems complicate the study of lateral root development. Here we describe a small-molecule screen in Arabidopsis thaliana that identified naxillin as what is to our knowledge the first non-auxin-like molecule that promotes root branching. By using naxillin as a chemical tool, we identified a new function for root cap–specific conversion of the auxin precursor indole-3-butyric acid into the active auxin indole-3-acetic acid and uncovered the involvement of the root cap in root branching. Delivery of an auxin precursor in peripheral tissues such as the root cap might represent an important mechanism shaping root architecture.",

author = "{De Rybel}, Bert and Dominique Audenaert and Wei Xuan and Paul Overvoorde and Strader, {Lucia C} and S. Kepinski and Rebecca Hoye and Ronald Brisbois and Boris Parizot and Steffen Vanneste and Xing Liu and Alison Gilday and Graham, {Ian Alexander} and Long Nguyen and Leentje Jansen and Njo, {Maria Fransisca} and Dirk Inze and Bonnie Bartel and Tom Beeckman",

year = "2012",

month = sep,

doi = "10.1038/nchembio.1044",

language = "English",

volume = "8",

pages = "798--805",

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De Rybel, B, Audenaert, D, Xuan, W, Overvoorde, P, Strader, LC, Kepinski, S, Hoye, R, Brisbois, R, Parizot, B, Vanneste, S, Liu, X, Gilday, A, Graham, IA, Nguyen, L, Jansen, L, Njo, MF, Inze, D, Bartel, B & Beeckman, T 2012, 'A role for the root cap in root branching revealed by the non-auxin probe naxillin.', NATURE CHEMICAL BIOLOGY, vol. 8, no. 9, pp. 798-805. https://doi.org/10.1038/nchembio.1044

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T1 - A role for the root cap in root branching revealed by the non-auxin probe naxillin.

AU - De Rybel, Bert

AU - Audenaert, Dominique

AU - Xuan, Wei

AU - Overvoorde, Paul

AU - Strader, Lucia C

AU - Kepinski, S.

AU - Hoye, Rebecca

AU - Brisbois, Ronald

AU - Parizot, Boris

AU - Vanneste, Steffen

AU - Liu, Xing

AU - Gilday, Alison

AU - Graham, Ian Alexander

AU - Nguyen, Long

AU - Jansen, Leentje

AU - Njo, Maria Fransisca

AU - Inze, Dirk

AU - Bartel, Bonnie

AU - Beeckman, Tom

PY - 2012/9

Y1 - 2012/9

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AB - he acquisition of water and nutrients by plant roots is a fundamental aspect of agriculture and strongly depends on root architecture. Root branching and expansion of the root system is achieved through the development of lateral roots and is to a large extent controlled by the plant hormone auxin. However, the pleiotropic effects of auxin or auxin-like molecules on root systems complicate the study of lateral root development. Here we describe a small-molecule screen in Arabidopsis thaliana that identified naxillin as what is to our knowledge the first non-auxin-like molecule that promotes root branching. By using naxillin as a chemical tool, we identified a new function for root cap–specific conversion of the auxin precursor indole-3-butyric acid into the active auxin indole-3-acetic acid and uncovered the involvement of the root cap in root branching. Delivery of an auxin precursor in peripheral tissues such as the root cap might represent an important mechanism shaping root architecture.

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DO - 10.1038/nchembio.1044

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