A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

S.M. Bromfield; D.K. Smith; P. Posocco; M. Fermeglia; S. Pricl; J. Rodríguez-López

doi:10.1039/c3cc41251b

A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

S.M. Bromfield, D.K. Smith, P. Posocco, M. Fermeglia, S. Pricl, J. Rodríguez-López

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum-using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.

Original language	English
Pages (from-to)	4830-4832
Journal	Chemical communications
Volume	49
Issue number	42
DOIs	https://doi.org/10.1039/c3cc41251b
Publication status	Published - 25 May 2013

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abstract = "We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum-using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.",

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AU - Posocco, P.

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AU - Pricl, S.

AU - Rodríguez-López, J.

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AB - We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum-using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.

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A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

Abstract

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