A Spotlight on Rad52 in Cyanidiophytina (Rhodophyta): A Relic in Algal Heritage

Angelo Del Mondo; Manuela Iovinella; Milena Petriccione; Angelina Nunziata; Seth Jon Davis; Diana Cioppa; Claudia Ciniglia

doi:10.3390/plants8020046

A Spotlight on Rad52 in Cyanidiophytina (Rhodophyta): A Relic in Algal Heritage

Angelo Del Mondo, Manuela Iovinella, Milena Petriccione, Angelina Nunziata, Seth Jon Davis, Diana Cioppa, Claudia Ciniglia

Research output: Contribution to journal › Article › peer-review

Abstract

RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences double-strand break repair and meiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports for the first time the presence of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of RAD52 protein in Cyanidiophyceae. Our findings support that RAD52 gene and protein have an ancient origin, though it has been subsequently lost in all green algae and land plants. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which – conversely to extant Rhodophyta - sexual mating was never observed.

Original language	English
Article number	46
Journal	Plants
Volume	8
Issue number	2
DOIs	https://doi.org/10.3390/plants8020046
Publication status	Published - 19 Feb 2019

Bibliographical note

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10.3390/plants8020046

Davis - Del Mondo et al
© 2019 by the authors
Accepted author manuscript, 648 KBLicence: CC BY

Cite this

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title = "A Spotlight on Rad52 in Cyanidiophytina (Rhodophyta): A Relic in Algal Heritage",

abstract = "RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences double-strand break repair and meiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports for the first time the presence of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of RAD52 protein in Cyanidiophyceae. Our findings support that RAD52 gene and protein have an ancient origin, though it has been subsequently lost in all green algae and land plants. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which – conversely to extant Rhodophyta - sexual mating was never observed.",

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AU - Del Mondo, Angelo

AU - Iovinella, Manuela

AU - Petriccione, Milena

AU - Nunziata, Angelina

AU - Davis, Seth Jon

AU - Cioppa, Diana

AU - Ciniglia, Claudia

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AB - RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences double-strand break repair and meiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports for the first time the presence of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of RAD52 protein in Cyanidiophyceae. Our findings support that RAD52 gene and protein have an ancient origin, though it has been subsequently lost in all green algae and land plants. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which – conversely to extant Rhodophyta - sexual mating was never observed.

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