Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study

Anand Manoharan; Peter J. Rayner; Wissam Iali; Michael J. Burns; V. Hugh Perry; Simon B. Duckett

doi:10.1002/cmdc.201700725

Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study

Anand Manoharan, Peter J. Rayner, Wissam Iali, Michael J. Burns, V. Hugh Perry, Simon B. Duckett^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.

Original language	English
Number of pages	9
Journal	ChemMedChem
Early online date	12 Dec 2017
DOIs	https://doi.org/10.1002/cmdc.201700725
Publication status	E-pub ahead of print - 12 Dec 2017

Bibliographical note

Keywords

Biocompatibility
Biphasic catalysis
Cytotoxicity
Hyperpolarization
SABRE

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10.1002/cmdc.201700725

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Accepted author manuscript, 2.12 MBLicence: CC BY
Manoharan_et_al-2018-ChemMedChem
© 2018, The Authors.
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Cite this

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title = "Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study",

abstract = "Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.",

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AU - Rayner, Peter J.

AU - Iali, Wissam

AU - Burns, Michael J.

AU - Perry, V. Hugh

AU - Duckett, Simon B.

PY - 2017/12/12

Y1 - 2017/12/12

N2 - Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.

AB - Production of a biocompatible hyperpolarized bolus for signal amplification by reversible exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to preclinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Herein, we exemplify the cytotoxicity of the SABRE approach using invitro cell assays. We conclude that the main cause of the observed toxicity is due to the SABRE catalyst. We therefore illustrate two catalyst removal methods: one involving deactivation and ion-exchange chromatography, and the second using biphasic catalysis. These routes produce a bolus suitable for future invivo study.

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KW - Biphasic catalysis

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KW - Hyperpolarization

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Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Hyperpolarisation using SABRE as a new tool for imaging

Cite this

Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Projects

Hyperpolarisation using SABRE as a new tool for imaging

Cite this