Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

S.A. Weller; D.E. Stead; J.P.W. Young

doi:10.1128/AEM.70.5.2779–2785.2004

Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

S.A. Weller, D.E. Stead, J.P.W. Young

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%).

Original language	English
Pages (from-to)	2779-2785
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	70
Issue number	5
DOIs	https://doi.org/10.1128/AEM.70.5.2779–2785.2004
Publication status	Published - 2004

Bibliographical note

Keywords

MICROBIAL IDENTIFICATION SYSTEM
TI-PLASMID
RHIZOBIUM-MELILOTI
TRANSFORMED PLANTS
TAQMAN PCR
TUMEFACIENS
STRAINS
RHIZOGENES
SEQUENCE
RADIOBACTER

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@article{d0d4cdbfdcab4b118adf73075e596c89,

title = "Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat",

abstract = "Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%).",

keywords = "MICROBIAL IDENTIFICATION SYSTEM, TI-PLASMID, RHIZOBIUM-MELILOTI, TRANSFORMED PLANTS, TAQMAN PCR, TUMEFACIENS, STRAINS, RHIZOGENES, SEQUENCE, RADIOBACTER",

author = "S.A. Weller and D.E. Stead and J.P.W. Young",

note = "Copyright {\textcopyright} 2004 American Society for Microbiology",

year = "2004",

doi = "10.1128/AEM.70.5.2779–2785.2004",

language = "English",

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T1 - Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

AU - Weller, S.A.

AU - Stead, D.E.

AU - Young, J.P.W.

PY - 2004

Y1 - 2004

N2 - Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%).

AB - Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%).

KW - MICROBIAL IDENTIFICATION SYSTEM

KW - TI-PLASMID

KW - RHIZOBIUM-MELILOTI

KW - TRANSFORMED PLANTS

KW - TAQMAN PCR

KW - TUMEFACIENS

KW - STRAINS

KW - RHIZOGENES

KW - SEQUENCE

KW - RADIOBACTER

U2 - 10.1128/AEM.70.5.2779–2785.2004

DO - 10.1128/AEM.70.5.2779–2785.2004

M3 - Article

SN - 0099-2240

VL - 70

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EP - 2785

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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ER -