Activation of the Non-canonical Inflammasome in Mouse and Human Cells

Jelena S Bezbradica; Rebecca C Coll; Dave Boucher

doi:10.1007/978-1-0716-2144-8_5

Activation of the Non-canonical Inflammasome in Mouse and Human Cells

Jelena S Bezbradica, Rebecca C Coll, Dave Boucher^*

^*Corresponding author for this work

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting.

Original language	English
Pages (from-to)	51-63
Number of pages	13
Journal	Methods in Molecular Biology
Volume	2459
DOIs	https://doi.org/10.1007/978-1-0716-2144-8_5
Publication status	Published - 25 Feb 2022

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10.1007/978-1-0716-2144-8_5

Cite this

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title = "Activation of the Non-canonical Inflammasome in Mouse and Human Cells",

abstract = "The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting.",

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