AePUb promoter length modulates gene expression in Aedes aegypti

Michelle Anderson; Philip Leftwich; Ray Wilson; Leonela Zusel Carabajal Paladino; Zach Adelman; Luke Stephen Alphey

doi:10.1038/s41598-023-47777-3

AePUb promoter length modulates gene expression in Aedes aegypti

Michelle Anderson, Philip Leftwich, Ray Wilson, Leonela Zusel Carabajal Paladino, Zach Adelman, Luke Stephen Alphey^*

^*Corresponding author for this work

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.

Original language	English
Article number	20352
Number of pages	7
Journal	Scientific Reports
Volume	13
DOIs	https://doi.org/10.1038/s41598-023-47777-3
Publication status	Published - 21 Nov 2023

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10.1038/s41598-023-47777-3Licence: CC BY

s41598-023-47777-3Final published version, 1.43 MBLicence: CC BY

Safe Genes Daisy Drives
Alphey, L. S., Anderson, M. & Ang Xin De, J.
1/09/17 → 31/08/19
Project: Other project › Project from former institution

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title = "AePUb promoter length modulates gene expression in Aedes aegypti",

abstract = "Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.",

author = "Michelle Anderson and Philip Leftwich and Ray Wilson and {Carabajal Paladino}, {Leonela Zusel} and Zach Adelman and Alphey, {Luke Stephen}",

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AU - Anderson, Michelle

AU - Leftwich, Philip

AU - Wilson, Ray

AU - Carabajal Paladino, Leonela Zusel

AU - Adelman, Zach

AU - Alphey, Luke Stephen

PY - 2023/11/21

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AB - Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.

U2 - 10.1038/s41598-023-47777-3

DO - 10.1038/s41598-023-47777-3

M3 - Article

SN - 2045-2322

VL - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 20352

ER -

AePUb promoter length modulates gene expression in Aedes aegypti

Abstract

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Safe Genes Daisy Drives

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