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alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis

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alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis. / Kumar, A.; Brannigan, J.A.; Moran, C.P.

In: Journal of Bacteriology, Vol. 186, No. 4, 02.2004, p. 1078-1083.

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Harvard

Kumar, A, Brannigan, JA & Moran, CP 2004, 'alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis', Journal of Bacteriology, vol. 186, no. 4, pp. 1078-1083. https://doi.org/10.1128/JB.186.4.1078-1083.2004

APA

Kumar, A., Brannigan, J. A., & Moran, C. P. (2004). alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis. Journal of Bacteriology, 186(4), 1078-1083. https://doi.org/10.1128/JB.186.4.1078-1083.2004

Vancouver

Kumar A, Brannigan JA, Moran CP. alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis. Journal of Bacteriology. 2004 Feb;186(4):1078-1083. https://doi.org/10.1128/JB.186.4.1078-1083.2004

Author

Kumar, A. ; Brannigan, J.A. ; Moran, C.P. / alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 4. pp. 1078-1083.

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@article{37edd2674042444d93bd3583b9409807,
title = "alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis",
abstract = "At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the second type includes the spoIIA promoter, which is used by RNA polymerase containing the secondary sigma factor sigma(H). Previous genetic analyses have identified specific amino acids in alpha-helix E of Spo0A that are important for activation of Spo0A-dependent, sigma(A)-dependent promoters. However, these amino acids are not required for activation of the sigma(H)-dependent spoIIA promoter. We now report the effects of additional single-amino-acid substitutions and the effects of deletions in alpha-helix E. The effects of alanine substitutions revealed one new position (239) in Spo0A that appears to be specifically required for activation of the sigma(A)-dependent promoters. Based on the effects of a deletion mutation, we suggest that alpha-helix E in Spo0A is not directly involved in interaction with sigma(H)-RNA polymerase.",
keywords = "RESPONSE REGULATOR, TRANSCRIPTION FACTOR, SPORULATION, PHOSPHORELAY, INITIATION, BINDING, REGION",
author = "A. Kumar and J.A. Brannigan and C.P. Moran",
note = "Copyright {\circledC} 2004 American Society for Microbiology",
year = "2004",
month = "2",
doi = "10.1128/JB.186.4.1078-1083.2004",
language = "English",
volume = "186",
pages = "1078--1083",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "4",

}

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TY - JOUR

T1 - alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis

AU - Kumar, A.

AU - Brannigan, J.A.

AU - Moran, C.P.

N1 - Copyright © 2004 American Society for Microbiology

PY - 2004/2

Y1 - 2004/2

N2 - At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the second type includes the spoIIA promoter, which is used by RNA polymerase containing the secondary sigma factor sigma(H). Previous genetic analyses have identified specific amino acids in alpha-helix E of Spo0A that are important for activation of Spo0A-dependent, sigma(A)-dependent promoters. However, these amino acids are not required for activation of the sigma(H)-dependent spoIIA promoter. We now report the effects of additional single-amino-acid substitutions and the effects of deletions in alpha-helix E. The effects of alanine substitutions revealed one new position (239) in Spo0A that appears to be specifically required for activation of the sigma(A)-dependent promoters. Based on the effects of a deletion mutation, we suggest that alpha-helix E in Spo0A is not directly involved in interaction with sigma(H)-RNA polymerase.

AB - At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the second type includes the spoIIA promoter, which is used by RNA polymerase containing the secondary sigma factor sigma(H). Previous genetic analyses have identified specific amino acids in alpha-helix E of Spo0A that are important for activation of Spo0A-dependent, sigma(A)-dependent promoters. However, these amino acids are not required for activation of the sigma(H)-dependent spoIIA promoter. We now report the effects of additional single-amino-acid substitutions and the effects of deletions in alpha-helix E. The effects of alanine substitutions revealed one new position (239) in Spo0A that appears to be specifically required for activation of the sigma(A)-dependent promoters. Based on the effects of a deletion mutation, we suggest that alpha-helix E in Spo0A is not directly involved in interaction with sigma(H)-RNA polymerase.

KW - RESPONSE REGULATOR

KW - TRANSCRIPTION FACTOR

KW - SPORULATION

KW - PHOSPHORELAY

KW - INITIATION

KW - BINDING

KW - REGION

U2 - 10.1128/JB.186.4.1078-1083.2004

DO - 10.1128/JB.186.4.1078-1083.2004

M3 - Article

VL - 186

SP - 1078

EP - 1083

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 4

ER -