By the same authors

From the same journal

Altering the energy landscape of virus self-assembly to generate kinetically trapped nanoparticles.

Research output: Contribution to journalArticle

Author(s)

  • Kevin Burns
  • Santanu Mukherjee
  • Thomas Keef
  • Jennifer M. Johnson
  • Adam Zlotnick

Department/unit(s)

Publication details

JournalBiomacromolecules
DatePublished - 1 Feb 2010
Issue number2
Volume11
Number of pages4
Pages (from-to)439-442
Original languageEnglish

Abstract

Controlling self-assembly is critical to the advancement of nanotechnology. A rugged or crenated assembly energy surface can redirect assembly off path. By using a defined starting point and an energy surface made rough by a strong association energy, we can impose entirely new assembly paths and products. Normally, the coat protein (CP) of the Cowpea Chlorotic Mottle Virus (CCMV) assembles into virus-like 28 nm diameter icosahedral particles. Here we have started with the coat protein trapped in a rod-like structure in complex with DNA. When these 17 nm diameter rods are placed under the same condition, low pH, that normally leads to assembly of 28 nm diameter particles, we instead obtain 17 nm capsids. The extrusion of all-pentamer capsids from the hexagonal lattice of the rod demonstrates the importance of the starting state for controlled assembly.

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