By the same authors

From the same journal

From the same journal

An ATP-binding cassette-type cysteine transporter in Campylobacter jejuni inferred from the structure of an extracytoplasmic solute receptor protein

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalMolecular Microbiology
DatePublished - Jul 2005
Issue number1
Volume57
Number of pages13
Pages (from-to)143-155
Original languageEnglish

Abstract

Campylobacter jejuni is a Gram-negative food-borne pathogen associated with gastroenteritis in humans as well as cases of the autoimmune disease Guillain Barre syndrome. C. jejuni is asaccharolytic because it lacks an active glycolytic pathway for the use of sugars as a carbon source. This suggests an increased reliance on amino acids as nutrients and indeed the genome sequence of this organism indicates the presence of a number of amino acid uptake systems. Cj0982, also known as CjaA, is a putative extracytoplasmic solute receptor for one such uptake system as well as a major surface antigen and vaccine candidate. The crystal structure of Cj0982 reveals a two-domain protein with density in the enclosed cavity between the domains that clearly defines the presence of a bound cysteine ligand. Fluorescence titration experiments were used to demonstrate that Cj0982 binds cysteine tightly and specifically with a K-d of similar to 10(-7) M consistent with a role as a receptor for a high- affinity transporter. These data imply that Cj0982 is the binding protein component of an ABC-type cysteine transporter system and that cysteine uptake is important in the physiology of C. jejuni.

Bibliographical note

© 2005 Blackwell Publishing Ltd. This is an electronic version of an article published in Molecular Microbiology: complete citation information for the final version of the paper, as published in the print edition of Molecular Microbiology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at www.blackwellpublishing.com/journal.asp?ref=0950-382Xor www.blackwell-synergy.com

    Research areas

  • NANOLITRE CRYSTALLIZATION EXPERIMENTS, ABC TRANSPORTERS, THERMOPHILIC CAMPYLOBACTERS, LIGAND SPECIFICITY, MOLECULAR GRAPHICS, BACILLUS-SUBTILIS, ESCHERICHIA-COLI, GENE, PATHOGEN, COLONIZATION

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations