An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites

TY - JOUR

T1 - An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites

AU - Patil, Samadhan B.

AU - Dheeman, Dharmendra S.

AU - Al-Rawhani, Mohammed A.

AU - Velugotla, Srinivas

AU - Nagy, Bence

AU - Cheah, Boon Chong

AU - Grant, James P.

AU - Accarino, Claudio

AU - Barrett, Michael P.

AU - Cumming, David R.S.

N1 - © 2018 The Authors.

PY - 2018/12/30

Y1 - 2018/12/30

N2 - Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.

AB - Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.

KW - CMOS

KW - Ischaemia

KW - Metabolites

KW - Multiplexed detection

KW - Photodiode

KW - Point-of-care detection

UR - http://www.scopus.com/inward/record.url?scp=85053815985&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2018.09.013

DO - 10.1016/j.bios.2018.09.013

M3 - Article

C2 - 30245326

AN - SCOPUS:85053815985

SN - 0956-5663

VL - 122

SP - 88

EP - 94

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -