An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites

Samadhan B. Patil*, Dharmendra S. Dheeman, Mohammed A. Al-Rawhani, Srinivas Velugotla, Bence Nagy, Boon Chong Cheah, James P. Grant, Claudio Accarino, Michael P. Barrett, David R.S. Cumming

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.

Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalBiosensors and Bioelectronics
Volume122
Early online date5 Sep 2018
DOIs
Publication statusPublished - 30 Dec 2018

Bibliographical note

© 2018 The Authors.

Keywords

  • CMOS
  • Ischaemia
  • Metabolites
  • Multiplexed detection
  • Photodiode
  • Point-of-care detection

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