By the same authors

From the same journal

From the same journal

An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalPlant Physiology
DatePublished - 2005
Issue number4
Volume138
Number of pages10
Pages (from-to)1842-1852
Original languageEnglish

Abstract

One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is involved in defense against potato virus X at the level of systemic spreading and in exclusion of the virus from the apical growing point. It has no effect on primary replication and cell-to-cell movement of the virus and does not contribute significantly to the formation of virus-derived small interfering (si) RNA in a fully established potato virus X infection. In grafting experiments, the RDR6 homolog was required for the ability of a cell to respond to, but not to produce or translocate, the systemic silencing signal. Taking these findings together, we suggest a model of virus defense in which RDR6 uses incoming silencing signal to generate double-stranded RNA precursors of secondary siRNA. According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves.

    Research areas

  • Base Sequence, Gene Expression Regulation, Plant, Gene Silencing, Genotype, Meristem, Molecular Sequence Data, Phenotype, Plant Leaves, Plant Proteins, Plants, Genetically Modified, Potexvirus, RNA Replicase, RNA, Plant, Sequence Homology, Amino Acid, Tobacco

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations