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An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal

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An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. / Schwach, Frank; Vaistij, Fabian E; Jones, Louise; Baulcombe, David C.

In: Plant Physiology, Vol. 138, No. 4, 2005, p. 1842-1852.

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Harvard

Schwach, F, Vaistij, FE, Jones, L & Baulcombe, DC 2005, 'An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal', Plant Physiology, vol. 138, no. 4, pp. 1842-1852. https://doi.org/10.1104/pp.105.063537

APA

Schwach, F., Vaistij, F. E., Jones, L., & Baulcombe, D. C. (2005). An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiology, 138(4), 1842-1852. https://doi.org/10.1104/pp.105.063537

Vancouver

Schwach F, Vaistij FE, Jones L, Baulcombe DC. An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiology. 2005;138(4):1842-1852. https://doi.org/10.1104/pp.105.063537

Author

Schwach, Frank ; Vaistij, Fabian E ; Jones, Louise ; Baulcombe, David C. / An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. In: Plant Physiology. 2005 ; Vol. 138, No. 4. pp. 1842-1852.

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@article{fb9affe27a0448fd9aff95b79c047509,
title = "An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal",
abstract = "One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is involved in defense against potato virus X at the level of systemic spreading and in exclusion of the virus from the apical growing point. It has no effect on primary replication and cell-to-cell movement of the virus and does not contribute significantly to the formation of virus-derived small interfering (si) RNA in a fully established potato virus X infection. In grafting experiments, the RDR6 homolog was required for the ability of a cell to respond to, but not to produce or translocate, the systemic silencing signal. Taking these findings together, we suggest a model of virus defense in which RDR6 uses incoming silencing signal to generate double-stranded RNA precursors of secondary siRNA. According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves.",
keywords = "Base Sequence, Gene Expression Regulation, Plant, Gene Silencing, Genotype, Meristem, Molecular Sequence Data, Phenotype, Plant Leaves, Plant Proteins, Plants, Genetically Modified, Potexvirus, RNA Replicase, RNA, Plant, Sequence Homology, Amino Acid, Tobacco",
author = "Frank Schwach and Vaistij, {Fabian E} and Louise Jones and Baulcombe, {David C}",
year = "2005",
doi = "10.1104/pp.105.063537",
language = "English",
volume = "138",
pages = "1842--1852",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - An RNA-dependent RNA Polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal

AU - Schwach, Frank

AU - Vaistij, Fabian E

AU - Jones, Louise

AU - Baulcombe, David C

PY - 2005

Y1 - 2005

N2 - One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is involved in defense against potato virus X at the level of systemic spreading and in exclusion of the virus from the apical growing point. It has no effect on primary replication and cell-to-cell movement of the virus and does not contribute significantly to the formation of virus-derived small interfering (si) RNA in a fully established potato virus X infection. In grafting experiments, the RDR6 homolog was required for the ability of a cell to respond to, but not to produce or translocate, the systemic silencing signal. Taking these findings together, we suggest a model of virus defense in which RDR6 uses incoming silencing signal to generate double-stranded RNA precursors of secondary siRNA. According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves.

AB - One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is involved in defense against potato virus X at the level of systemic spreading and in exclusion of the virus from the apical growing point. It has no effect on primary replication and cell-to-cell movement of the virus and does not contribute significantly to the formation of virus-derived small interfering (si) RNA in a fully established potato virus X infection. In grafting experiments, the RDR6 homolog was required for the ability of a cell to respond to, but not to produce or translocate, the systemic silencing signal. Taking these findings together, we suggest a model of virus defense in which RDR6 uses incoming silencing signal to generate double-stranded RNA precursors of secondary siRNA. According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves.

KW - Base Sequence

KW - Gene Expression Regulation, Plant

KW - Gene Silencing

KW - Genotype

KW - Meristem

KW - Molecular Sequence Data

KW - Phenotype

KW - Plant Leaves

KW - Plant Proteins

KW - Plants, Genetically Modified

KW - Potexvirus

KW - RNA Replicase

KW - RNA, Plant

KW - Sequence Homology, Amino Acid

KW - Tobacco

U2 - 10.1104/pp.105.063537

DO - 10.1104/pp.105.063537

M3 - Article

C2 - 16040651

VL - 138

SP - 1842

EP - 1852

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -