Analysis and validation of overall N-glycan conformation in Privateer

Jordan S Dialpuri, Haroldas Bagdonas, Mihaela Atanasova, Lucy C Schofield, Maarten L Hekkelman, Robbie P Joosten, Jon Agirre

Research output: Contribution to journalArticlepeer-review


The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein. These contributions are dependent on the composition and overall conformation of the glycans. The Privateer software allows structural biologists to evaluate and improve the atomic structures of carbohydrates, including N-glycans; this software has recently been extended to check glycan composition through the use of glycomics data. Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.

Original languageEnglish
Pages (from-to)462–472
Number of pages11
JournalActa crystallographica. Section D, Structural biology
Publication statusPublished - 1 Jun 2023

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