Analysis and validation of overall N-glycan conformation in Privateer

Jordan S Dialpuri; Haroldas Bagdonas; Mihaela Atanasova; Lucy C Schofield; Maarten L Hekkelman; Robbie P Joosten; Jon Agirre

doi:10.1107/S2059798323003510

Analysis and validation of overall N-glycan conformation in Privateer

Jordan S Dialpuri, Haroldas Bagdonas, Mihaela Atanasova, Lucy C Schofield, Maarten L Hekkelman, Robbie P Joosten, Jon Agirre

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein. These contributions are dependent on the composition and overall conformation of the glycans. The Privateer software allows structural biologists to evaluate and improve the atomic structures of carbohydrates, including N-glycans; this software has recently been extended to check glycan composition through the use of glycomics data. Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.

Original language	English
Pages (from-to)	462–472
Number of pages	11
Journal	Acta crystallographica. Section D, Structural biology
Volume	D79
DOIs	https://doi.org/10.1107/S2059798323003510
Publication status	Published - 1 Jun 2023

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Analysis and validation of overall N-glycan conformation in PrivateerFinal published version, 1.45 MBLicence: CC BY

Cite this

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AU - Dialpuri, Jordan S

AU - Bagdonas, Haroldas

AU - Atanasova, Mihaela

AU - Schofield, Lucy C

AU - Hekkelman, Maarten L

AU - Joosten, Robbie P

AU - Agirre, Jon

PY - 2023/6/1

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AB - The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein. These contributions are dependent on the composition and overall conformation of the glycans. The Privateer software allows structural biologists to evaluate and improve the atomic structures of carbohydrates, including N-glycans; this software has recently been extended to check glycan composition through the use of glycomics data. Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.

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JO - Acta crystallographica. Section D, Structural biology

JF - Acta crystallographica. Section D, Structural biology

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