By the same authors

From the same journal

Analysis of chemokine receptor endocytosis and intracellular trafficking

Research output: Contribution to journalArticlepeer-review



Publication details

JournalMethods in enzymology
DatePublished - 2009
Issue numberA
Number of pages21
Pages (from-to)357-377
Original languageEnglish


Chemokine receptors are G protein-coupled receptors (GPCRs) that, through their ability to regulate chemotaxis by responding to small chemoattractant peptides termed chemokines, are involved in the development, maintenance, and functional activities of the immune system. In addition, members of the chemokine receptor family have been implicated in a number of other physiological and pathological processes, including human immunodeficiency virus infection and malaria. These activities are dependent on receptor expression at the cell surface and cellular events that reduce the cell-surface expression of chemokine receptors can abrogate these activities. Moreover, internalization of chemokine receptors by endocytosis is necessary for both receptor degradation and recycling, key regulatory processes that determine cell-surface expression levels. Here we provide detailed methods for the quantitative analysis of CCR5 endocytosis and recycling by flow cytometry, as well as fluorescence and electron microscopic procedures to analyze the endocytosis and intracellular trafficking of CCR5 by immunolabeling of cells or cryosections. In principle, the same approaches can be used for analyzing other chemokine receptors and other GPCR or non-GPCR cell-surface proteins.

    Research areas

  • Animals, Blotting, Western, CHO Cells, Cricetinae, Cricetulus, Endocytosis, Flow Cytometry, Humans, Microscopy, Electron, Microscopy, Fluorescence, Protein Transport, Receptors, Chemokine

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