Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging

Damien Vincke; Rebecca Miller; Edith Stassart; Marcel Otte; Pierre Dardenne; Matthew Collins; Keith Wilkinson; John Stewart; Vincent Baeten; Juan Antonio Fernández Pierna

doi:10.1016/j.talanta.2014.02.044

Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging

Damien Vincke, Rebecca Miller, Edith Stassart, Marcel Otte, Pierre Dardenne, Matthew Collins, Keith Wilkinson, John Stewart, Vincent Baeten, Juan Antonio Fernández Pierna

Archaeology

Research output: Contribution to journal › Article › peer-review

Abstract

The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al׳Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.

Original language	English
Pages (from-to)	181-8
Number of pages	8
Journal	Talanta
Volume	125
DOIs	https://doi.org/10.1016/j.talanta.2014.02.044
Publication status	Published - 1 Jul 2014

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10.1016/j.talanta.2014.02.044

http://www.sciencedirect.com/science/article/pii/S0039914014001428

Cite this

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title = "Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging",

abstract = "The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al׳Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.",

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AU - Vincke, Damien

AU - Miller, Rebecca

AU - Stassart, Edith

AU - Otte, Marcel

AU - Dardenne, Pierre

AU - Collins, Matthew

AU - Wilkinson, Keith

AU - Stewart, John

AU - Baeten, Vincent

AU - Fernández Pierna, Juan Antonio

PY - 2014/7/1

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N2 - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al׳Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.

AB - The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al׳Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for subsequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.

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