Abstract
Biomimetic scaffolds, delivered to the patient with or without cell loading, can offer improved treatments for a range of damaged or diseased tissues. In this work we evaluated the capacity of a cross-linked collagen-based implant material (Permacol (TM)) to support the survival and growth of multipotent stromal cells or mesenchymal stem cells (MSCs) for potential applications in soft-tissue reconstruction and compared it to uncross-linked collagen. We showed, using confocal imaging, histology, quantitative analysis of seeding efficiencies by the MTT assay and Live/Dead analysis, that Permacol (TM) supported the adherence, growth and survival of MSCs preserving a healthy cell phenotype. MSCs infiltrated both matrices, although migration rates were significantly higher on uncross-linked matrices for some donors. Our work demonstrates that Permacol (TM) could maintain human MSCs in long-term culture supporting its use as a cell-seeded biomaterial for durable and strong repair in tissue reconstruction.
| Original language | English |
|---|---|
| Pages (from-to) | 261-276 |
| Journal | Bio-Medical materials and engineering |
| Volume | 22 |
| Issue number | 5 |
| Early online date | 28 Sept 2012 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- Mesenchymal human stem cells
- collagen implant material
- regenerative
- surgical
- PORCINE DERMAL COLLAGEN
- SOFT-TISSUE RECONSTRUCTION
- ADIPOSE-DERIVED STEM
- HERNIA REPAIR
- RAT MODEL
- IN-VITRO
- MATRIX
- LINKING
- PERMACOL(TM)
- SCAFFOLDS