Ancient genomics

Clio Der Sarkissian; Morten E. Allentoft; María C. Ávila-Arcos; Ross Barnett; Paula F Campos; Enrico Cappellini; Luca Ermini; Ruth Fernández; Rute A R da Fonseca; Aurélien Ginolhac; Anders J. Hansen; Hákon Jónsson; Thorfinn Sand Korneliussen; Ashot Margaryan; Michael D. Martin; J. Víctor Moreno-Mayar; Maanasa Raghavan; Morten Rasmussen; Marcela Sandoval Velasco; Hannes Schroeder; Mikkel Schubert; Andaine Seguin-Orlando; Nathan Wales; M. Thomas P. Gilbert; Eske Willerslev; Ludovic Orlando

doi:10.1098/rstb.2013.0387

Ancient genomics

Clio Der Sarkissian, Morten E. Allentoft, María C. Ávila-Arcos, Ross Barnett, Paula F Campos, Enrico Cappellini, Luca Ermini, Ruth Fernández, Rute A R da Fonseca, Aurélien Ginolhac, Anders J. Hansen, Hákon Jónsson, Thorfinn Sand Korneliussen, Ashot Margaryan, Michael D. Martin, J. Víctor Moreno-Mayar, Maanasa Raghavan, Morten Rasmussen, Marcela Sandoval Velasco, Hannes SchroederMikkel Schubert, Andaine Seguin-Orlando, Nathan Wales, M. Thomas P. Gilbert, Eske Willerslev, Ludovic Orlando^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The past decade has witnessed a revolution in ancient DNA (aDNA) research. Although the field's focus was previously limited to mitochondrial DNA and a few nuclear markers, whole genome sequences from the deep past can now be retrieved. This breakthrough is tightly connected to the massive sequence throughput of next generation sequencing platforms and the ability to target short and degraded DNA molecules. Many ancient specimens previously unsuitable for DNA analyses because of extensive degradation can now successfully be used as source materials. Additionally, the analytical power obtained by increasing the number of sequence reads to billions effectively means that contamination issues that have haunted aDNA research for decades, particularly in human studies, can now be efficiently and confidently quantified. At present, whole genomes have been sequenced from ancient anatomically modern humans, archaic hominins, ancient pathogens and megafaunal species. Those have revealed important functional and phenotypic information, as well as unexpected adaptation, migration and admixture patterns. As such, the field of aDNA has entered the new era of genomics and has provided valuable information when testing specific hypotheses related to the past.

Original language	English
Number of pages	12
Journal	Philosophical Transactions Of The Royal Society Of London Series B - Biological Sciences
Volume	370
Issue number	1660
DOIs	https://doi.org/10.1098/rstb.2013.0387
Publication status	Published - 19 Jan 2015

Keywords

Ancient DNA
Genomics
Next generation sequencing

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10.1098/rstb.2013.0387

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Der Sarkissian, C., Allentoft, M. E., Ávila-Arcos, M. C., Barnett, R., Campos, P. F., Cappellini, E., Ermini, L., Fernández, R., da Fonseca, R. A. R., Ginolhac, A., Hansen, A. J., Jónsson, H., Korneliussen, T. S., Margaryan, A., Martin, M. D., Víctor Moreno-Mayar, J., Raghavan, M., Rasmussen, M., Velasco, M. S., ... Orlando, L. (2015). Ancient genomics. Philosophical Transactions Of The Royal Society Of London Series B - Biological Sciences, 370(1660). https://doi.org/10.1098/rstb.2013.0387

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abstract = "The past decade has witnessed a revolution in ancient DNA (aDNA) research. Although the field's focus was previously limited to mitochondrial DNA and a few nuclear markers, whole genome sequences from the deep past can now be retrieved. This breakthrough is tightly connected to the massive sequence throughput of next generation sequencing platforms and the ability to target short and degraded DNA molecules. Many ancient specimens previously unsuitable for DNA analyses because of extensive degradation can now successfully be used as source materials. Additionally, the analytical power obtained by increasing the number of sequence reads to billions effectively means that contamination issues that have haunted aDNA research for decades, particularly in human studies, can now be efficiently and confidently quantified. At present, whole genomes have been sequenced from ancient anatomically modern humans, archaic hominins, ancient pathogens and megafaunal species. Those have revealed important functional and phenotypic information, as well as unexpected adaptation, migration and admixture patterns. As such, the field of aDNA has entered the new era of genomics and has provided valuable information when testing specific hypotheses related to the past.",

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Der Sarkissian, C, Allentoft, ME, Ávila-Arcos, MC, Barnett, R, Campos, PF, Cappellini, E, Ermini, L, Fernández, R, da Fonseca, RAR, Ginolhac, A, Hansen, AJ, Jónsson, H, Korneliussen, TS, Margaryan, A, Martin, MD, Víctor Moreno-Mayar, J, Raghavan, M, Rasmussen, M, Velasco, MS, Schroeder, H, Schubert, M, Seguin-Orlando, A, Wales, N, Gilbert, MTP, Willerslev, E & Orlando, L 2015, 'Ancient genomics', Philosophical Transactions Of The Royal Society Of London Series B - Biological Sciences, vol. 370, no. 1660. https://doi.org/10.1098/rstb.2013.0387

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T1 - Ancient genomics

AU - Der Sarkissian, Clio

AU - Allentoft, Morten E.

AU - Ávila-Arcos, María C.

AU - Barnett, Ross

AU - Campos, Paula F

AU - Cappellini, Enrico

AU - Ermini, Luca

AU - Fernández, Ruth

AU - da Fonseca, Rute A R

AU - Ginolhac, Aurélien

AU - Hansen, Anders J.

AU - Jónsson, Hákon

AU - Korneliussen, Thorfinn Sand

AU - Margaryan, Ashot

AU - Martin, Michael D.

AU - Víctor Moreno-Mayar, J.

AU - Raghavan, Maanasa

AU - Rasmussen, Morten

AU - Velasco, Marcela Sandoval

AU - Schroeder, Hannes

AU - Schubert, Mikkel

AU - Seguin-Orlando, Andaine

AU - Wales, Nathan

AU - Gilbert, M. Thomas P.

AU - Willerslev, Eske

AU - Orlando, Ludovic

PY - 2015/1/19

Y1 - 2015/1/19

N2 - The past decade has witnessed a revolution in ancient DNA (aDNA) research. Although the field's focus was previously limited to mitochondrial DNA and a few nuclear markers, whole genome sequences from the deep past can now be retrieved. This breakthrough is tightly connected to the massive sequence throughput of next generation sequencing platforms and the ability to target short and degraded DNA molecules. Many ancient specimens previously unsuitable for DNA analyses because of extensive degradation can now successfully be used as source materials. Additionally, the analytical power obtained by increasing the number of sequence reads to billions effectively means that contamination issues that have haunted aDNA research for decades, particularly in human studies, can now be efficiently and confidently quantified. At present, whole genomes have been sequenced from ancient anatomically modern humans, archaic hominins, ancient pathogens and megafaunal species. Those have revealed important functional and phenotypic information, as well as unexpected adaptation, migration and admixture patterns. As such, the field of aDNA has entered the new era of genomics and has provided valuable information when testing specific hypotheses related to the past.

AB - The past decade has witnessed a revolution in ancient DNA (aDNA) research. Although the field's focus was previously limited to mitochondrial DNA and a few nuclear markers, whole genome sequences from the deep past can now be retrieved. This breakthrough is tightly connected to the massive sequence throughput of next generation sequencing platforms and the ability to target short and degraded DNA molecules. Many ancient specimens previously unsuitable for DNA analyses because of extensive degradation can now successfully be used as source materials. Additionally, the analytical power obtained by increasing the number of sequence reads to billions effectively means that contamination issues that have haunted aDNA research for decades, particularly in human studies, can now be efficiently and confidently quantified. At present, whole genomes have been sequenced from ancient anatomically modern humans, archaic hominins, ancient pathogens and megafaunal species. Those have revealed important functional and phenotypic information, as well as unexpected adaptation, migration and admixture patterns. As such, the field of aDNA has entered the new era of genomics and has provided valuable information when testing specific hypotheses related to the past.

KW - Ancient DNA

KW - Genomics

KW - Next generation sequencing

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U2 - 10.1098/rstb.2013.0387

DO - 10.1098/rstb.2013.0387

M3 - Article

C2 - 25487338

AN - SCOPUS:84916210429

SN - 0962-8436

VL - 370

JO - Philosophical Transactions Of The Royal Society Of London Series B - Biological Sciences

JF - Philosophical Transactions Of The Royal Society Of London Series B - Biological Sciences

IS - 1660

ER -

Ancient genomics

Abstract

Keywords

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