Anomalous pH-gradient in Ampholyte Solution

L.V Sakharova, Vladimir A. Vladimirov, M.Yu. Zhukov

Research output: Working paper


A mathematical model describing a steady pH-gradient in the solution of ampholytes in water has been studied with the use of analytical, asymptotic, and numerical methods. We show that at the large values of an electric current a concentration distribution takes the form of a piecewise constant function that is drastically di.erent from a classical Gaussian form. The correspondent pH-gradient takes a stepwise form, instead of being a linear function. A discovered anomalous pH-gradient can crucially a.ect the understanding of an isoelectric focusing process.
Original languageEnglish
Number of pages6
Publication statusPublished - 21 Feb 2009


  • Fluid Dynamics
  • Electrophoresis;
  • pH-gradient;
  • isoelectrofuocusing;

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