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A mathematical model describing a steady pH-gradient in the solution of ampholytes in water has been studied with the use of analytical, asymptotic, and numerical methods. We show that at the large values of an electric current a concentration distribution takes the form of a piecewise constant function that is drastically di.erent from a classical Gaussian form. The correspondent pH-gradient takes a stepwise form, instead of being a linear function. A discovered anomalous pH-gradient can crucially a.ect the understanding of an isoelectric focusing process.
|Number of pages||6|
|Publication status||Published - 21 Feb 2009|
- Fluid Dynamics