By the same authors

From the same journal

Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

Department/unit(s)

Publication details

JournalBiotechnology and bioengineering
DatePublished - 5 Sep 2004
Issue number5
Volume87
Number of pages9
Pages (from-to)623-631
Original languageEnglish

Abstract

Regioselectivity of glycosyltransferases offers an important means to overcome the limitations of chemical synthesis of small molecule glycosides. In this study we explore a large multigene family of UDP-glucose:glycosyltransferases of Arabidopsis for their potential as novel biocatalysts for in vitro synthesis and whole-cell catalysis. We used quercetin as a substrate for this study because the flavonol and its glycosides have important medicinal properties and the metabolite provides a complex structure for regioselective glucosylation. We analyzed the activity of 91 recombinant enzymes for in vitro activity toward quercetin and discovered 29 that are capable of glucosylating the substrate. We demonstrate the first enzymic synthesis of a range of glucosides in vitro, including the 3-O-, 7-O-, 3'-O-, and 4'-O-monoglucosides, 3,7-di-O-glucoside, and 7,3'-di-O-glucoside. We also show that the regioselectivity of glucosylation can be maintained when the enzymes are used as whole-cell biocatalysts in Escherichia coli. (C) 2004 Wiley Periodicals, Inc.

    Research areas

  • glycosyltransferases, regioselective glucosylation, biocatalysis, MULTIGENE FAMILY, ALLIUM-CEPA, IN-VIVO, GLUCOSYLTRANSFERASE, GLYCOSIDES, ABSORPTION, METABOLISM, FLAVONOIDS, THALIANA, CLONING

Projects

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations