Arbuscular mycorrhizal dynamics in a chronosequence of Caragana korshinskii plantations

TY - JOUR

T1 - Arbuscular mycorrhizal dynamics in a chronosequence of Caragana korshinskii plantations

AU - Liu, Yongjun

AU - He, Lei

AU - An, Lizhe

AU - Helgason, Thorunn

AU - Feng, Huyuan

PY - 2009/1

Y1 - 2009/1

N2 - Arbuscular mycorrhizal (AM) fungi in a chronosequence of 5-42-year-old Caragana korshinskii plantations in the semi-arid Loess Plateau region of northwestern China were investigated. AM fungi colonization, spore diversity and PCR-denatured gradient gel electrophoresis-based AM fungal SSU rRNA gene sequences were analyzed. AM fungi colonization [measured as the percent of root length (%RLC), vesicular (%VC) and arbuscular (%AC) colonization] and spore density were significantly correlated with sampling month, but not with plant age, except for %RLC. The percent of vesicular colonization was negatively correlated with soil total nitrogen and organic carbon, and spore density was negatively correlated with soil moisture and available phosphorus. Ten distinguishable AM fungal spore morphotypes, nine Glomus and one Scutellospora species, were found. Nine AM fungal Glomus phylotypes were identified by sequencing, but at each sampling time only four to six AM fungal phylotypes were detected. The AM fungal community was significantly seasonal, whereas the AM fungal species richness did not increase with plantation age. A significant change in AM fungal colonization and community composition over an annual cycle was observed in this study, and our results suggest that the changes of AM are the product of the interaction between host phenology, soil characteristics and habitat. Understanding these interactions is essential if habitat restoration is to be effective.

AB - Arbuscular mycorrhizal (AM) fungi in a chronosequence of 5-42-year-old Caragana korshinskii plantations in the semi-arid Loess Plateau region of northwestern China were investigated. AM fungi colonization, spore diversity and PCR-denatured gradient gel electrophoresis-based AM fungal SSU rRNA gene sequences were analyzed. AM fungi colonization [measured as the percent of root length (%RLC), vesicular (%VC) and arbuscular (%AC) colonization] and spore density were significantly correlated with sampling month, but not with plant age, except for %RLC. The percent of vesicular colonization was negatively correlated with soil total nitrogen and organic carbon, and spore density was negatively correlated with soil moisture and available phosphorus. Ten distinguishable AM fungal spore morphotypes, nine Glomus and one Scutellospora species, were found. Nine AM fungal Glomus phylotypes were identified by sequencing, but at each sampling time only four to six AM fungal phylotypes were detected. The AM fungal community was significantly seasonal, whereas the AM fungal species richness did not increase with plantation age. A significant change in AM fungal colonization and community composition over an annual cycle was observed in this study, and our results suggest that the changes of AM are the product of the interaction between host phenology, soil characteristics and habitat. Understanding these interactions is essential if habitat restoration is to be effective.

KW - arbuscular mycorrhiza

KW - community

KW - Caragana korshinskii

KW - denaturing gradient gel electrophoresis (DGGE)

KW - succession

KW - seasonal dynamics

KW - HYACINTHOIDES-NON-SCRIPTA

KW - FUNGAL COMMUNITIES

KW - SEMINATURAL GRASSLAND

KW - MOLECULAR DIVERSITY

KW - SEASONAL DYNAMICS

KW - TROPICAL FOREST

KW - PLANTS

KW - ECOSYSTEM

KW - GRADIENT

KW - ROOTS

UR - http://www.scopus.com/inward/record.url?scp=57449098651&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6941.2008.00597.x

DO - 10.1111/j.1574-6941.2008.00597.x

M3 - Article

SN - 0168-6496

VL - 67

SP - 81

EP - 92

JO - FEMS MICROBIOLOGY ECOLOGY

JF - FEMS MICROBIOLOGY ECOLOGY

IS - 1

ER -