Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes

Elizabeth M Hill; Lynne A. Robinson; Ali Abdul-Sada; Adam J. Vanbergen; Angela Hodge; Susan E  Hartley

doi:10.1007/s10886-017-0921-1

Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes

Elizabeth M Hill, Lynne A. Robinson, Ali Abdul-Sada, Adam J. Vanbergen, Angela Hodge, Susan E Hartley

Research output: Contribution to journal › Article › peer-review

Abstract

Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms.

Original language	English
Pages (from-to)	198-208
Number of pages	11
Journal	Journal of chemical ecology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1007/s10886-017-0921-1
Publication status	Published - 2 Feb 2018

Bibliographical note

Keywords

Above-belowground interactions
Arbuscular mycorrhizal symbiosis
Blumenols
Metabolomics
Microbiome
Phytobiome
Pyrrolizidine alkaloids
Rhizophagus irregularis
Senecio jacobaea
Symbiosis
Plant Roots/physiology
Senecio/physiology
Pyrrolizidine Alkaloids/metabolism
Metabolome
Biomass
Mycorrhizae/physiology
Glomeromycota/physiology
Plant Shoots/physiology

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10.1007/s10886-017-0921-1

Hartley -FINAL REVISION JOCE-D-17-00277_R1Accepted author manuscript, 1.44 MB
10.1007%2Fs10886-017-0921-1
© The Author(s) 2018
Final published version, 805 KBLicence: CC BY

Cite this

@article{6078cf7e3c3d4da4a732e3ca4a439592,

title = "Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes",

abstract = "Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms.",

keywords = "Above-belowground interactions, Arbuscular mycorrhizal symbiosis, Blumenols, Metabolomics, Microbiome, Phytobiome, Pyrrolizidine alkaloids, Rhizophagus irregularis, Senecio jacobaea, Symbiosis, Plant Roots/physiology, Senecio/physiology, Pyrrolizidine Alkaloids/metabolism, Metabolome, Biomass, Mycorrhizae/physiology, Glomeromycota/physiology, Plant Shoots/physiology",

author = "Hill, {Elizabeth M} and Robinson, {Lynne A.} and Ali Abdul-Sada and Vanbergen, {Adam J.} and Angela Hodge and Hartley, {Susan E}",

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T2 - Effects of Colonisation on Aboveground and Belowground Metabolomes

AU - Hill, Elizabeth M

AU - Robinson, Lynne A.

AU - Abdul-Sada, Ali

AU - Vanbergen, Adam J.

AU - Hodge, Angela

AU - Hartley, Susan E

N1 - © The Author(s) 2018

PY - 2018/2/2

Y1 - 2018/2/2

N2 - Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms.

AB - Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms.

KW - Above-belowground interactions

KW - Arbuscular mycorrhizal symbiosis

KW - Blumenols

KW - Metabolomics

KW - Microbiome

KW - Phytobiome

KW - Pyrrolizidine alkaloids

KW - Rhizophagus irregularis

KW - Senecio jacobaea

KW - Symbiosis

KW - Plant Roots/physiology

KW - Senecio/physiology

KW - Pyrrolizidine Alkaloids/metabolism

KW - Metabolome

KW - Biomass

KW - Mycorrhizae/physiology

KW - Glomeromycota/physiology

KW - Plant Shoots/physiology

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U2 - 10.1007/s10886-017-0921-1

DO - 10.1007/s10886-017-0921-1

M3 - Article

C2 - 29392532

SN - 0098-0331

VL - 44

SP - 198

EP - 208

JO - Journal of chemical ecology

JF - Journal of chemical ecology

IS - 2

ER -

Arbuscular Mycorrhizal Fungi and Plant Chemical Defence: Effects of Colonisation on Aboveground and Belowground Metabolomes

Abstract

Bibliographical note

Keywords

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Angela Hodge

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